UNG (Uracil-DNA Glycosylase)

Uracil-DNA Glycosylase (UNG or UDG) is derived from recombinant cloning and expression of Escherichia coli, with a molecular weight of 25 kDa. It can catalyze the release of free uracil in single-stranded and double-stranded DNA containing uracil, and has no activity on RNA. It can be used to prevent contamination caused by PCR amplification products. Its principle is based on: if dUTP is substituted for dTTP in PCR reaction to form PCR amplification products containing dU bases, this enzyme can selectively break the glycosidic bond of U bases in single-stranded and double-stranded DNA, degrading the PCR amplification product.

Product Specification:

Concentration: 1U/μL

Lyophilization: Yes

Storage Buffer:

20 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, Stabilizer, 50% Glycerol.

Features:

- Digest dUTP template at 50℃

- Inactivated when heated at 95°C

• E01 说明书

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