This reagent kit uses LAMP technology and is designed to target the specific sequence with 4-6 specific LAMP primers. It utilizes Bst 2.0 HS isothermal amplification enzyme to recognize 6-8 independent regions of the target sequence, generating double-stranded DNA through isothermal amplification. During the amplification process, pyrophosphate ions are produced, leading to a decrease in pH. Therefore, the color change of the pH indicator can be used to interpret the results. The reagent kit appears purple-red before the reaction, with the positive wells turning yellow and the negative wells maintaining the purple-red color after the reaction.
Product Specification:
Reaction Temperature: 60~65℃,30~60min (After the reaction is completed, the results should be interpreted after cooling to room temperature.)
Lyophilization: Yes
Reagent Compositions
1、Bst2.0 HS (8 U/µL)
2、2×LAMP Premix Buffer (RY)
Intended Use
This kit uses a dye-based isothermal amplification, which can be used for qualitative detection of DNA in samples (such as viruses).
PM05 说明书
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