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BspQ I Restriction Enzyme

BspQ I Restriction Enzyme

  • Item number:BP-E09
  • Overview
  • experimental

This product is a recombinant protein derived from Escherichia coli expression of the BspQI gene from Bacillus sphaericus, which encodes a type IIS restriction endonuclease. It is primarily used for the linearization of in vitro transcription templates, by digesting plasmids to prepare linearized DNA fragments containing poly(A/T/G/C) ends. The recognition sequence and cutting site are as follows:

5'···GCTCTTC(N)↓···3'

3'···CGAGAAG(NNNN)↑···5'

 

Product Spec.:

10 U/μL

 

Standard of Quality Control:

1.    Solution is clear and transparent, with no visible foreign particles.

2.    Protein purity is ≥95%.

3.    No detectable DNase or RNase activity.

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