This product is a recombinant protein derived from Escherichia coli expression of the BspQI gene from Bacillus sphaericus, which encodes a type IIS restriction endonuclease. It is primarily used for the linearization of in vitro transcription templates, by digesting plasmids to prepare linearized DNA fragments containing poly(A/T/G/C) ends. The recognition sequence and cutting site are as follows:
5'···GCTCTTC(N)↓···3'
3'···CGAGAAG(NNNN)↑···5'
Product Spec.:
10 U/μL
Standard of Quality Control:
1. Solution is clear and transparent, with no visible foreign particles.
2. Protein purity is ≥95%.
3. No detectable DNase or RNase activity.
BP-E09 说明书
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