A fast, probe-based 2× qPCR master mix with hot-start DNA polymerase and UNG–dUTP carryover prevention for reliable real-time PCR quantification. The optimized buffer supports rapid cycling (protocol-dependent, ~30 min) and multiplex assays. Compatible with most real-time PCR instruments (e.g., Applied Biosystems™, Bio-Rad™, Roche™, Eppendorf™).
Fast cycling ready: optimized for rapid probe-based qPCR workflows, delivering results in ~30 minutes (protocol-dependent).
Hot-start DNA polymerase: high specificity and robust amplification with reduced non-specific products.
Multiplex compatible buffer system: improved performance in multiplex probe qPCR (optimize primers/probes as needed).
UNG–dUTP carryover prevention: helps minimize PCR carryover contamination and reduces the risk of false positives.
Stable and easy to use: 2× premix format, simply add primers/probe + template; consistent performance across runs.
Flexible formulation options: available in liquid (with glycerol) and glycerol-free formats for different manufacturing and storage needs.
| Item | Description |
|---|---|
| Master Mix | 2× FastAmpli All-in-One qPCR Master Mix with UNG |
| Included components | PCR buffer, MgCl2, dNTPs (incl. dUTP), hot-start DNA polymerase, UNG, stabilizers |
| Optional (formulation-dependent) | Lyoprotectants (for lyophilization-ready formats) |
Note: Exact composition may vary by formulation and is subject to change without notice.
| Condition | Recommendation |
|---|---|
| Shipping | Ship on ice packs (2–8°C) or dry ice (≤ −20°C), depending on transit time. |
| Storage | Store at −20°C. For frequent use, aliquot to minimize freeze–thaw cycles. |
| Handling | Thaw completely on ice, mix gently, and briefly spin down before use. |
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