Robustart Fast Taq DNA Polymerase-V3 is a hot-start rapid amplification Taq enzyme optimized for freeze-dried reagent systems. It effectively suppresses non-specific amplification caused by primer misannealing or primer-dimer formation during PCR setup and amplification, delivering high specificity and improved performance for low-concentration templates. With good system adaptability, it provides stable amplification results in different PCR reaction types and is suitable for multiplex PCR amplification.
| No. | Component | Notes |
|---|---|---|
| 1 | 5 U/μL Robustart Fast Taq DNA Polymerase-V3 | — |
| 2 | 10× PCR Buffer II (Mg2+-free) | Optional |
| 3 | 25 mM MgCl2 | Optional |
Note: The 10× PCR Buffer II (Mg2+-free) does not contain dNTPs or Mg2+. Please add dNTPs and MgCl2 when preparing the reaction system.
| Condition | Recommendation |
|---|---|
| Shipping | Ship on dry ice. |
| Storage | Store at −20 ± 5°C. |
| Handling | Mix thoroughly before use and avoid repeated freeze–thaw cycles. |
Experimental validation data will be updated soon.
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