Generated by Rank Math SEO, this is an llms.txt file designed to help LLMs better understand and index this website. # Zhuhai Biori Biotechnology Co., Ltd.: Zhuhai Biori Biotechnology Co., Ltd. ## Sitemaps [XML Sitemap](https://biori-en.com/sitemap_index.xml): Includes all crawlable and indexable pages. ## Posts - [Blood-Based Molecular Profiling Reagent Strategy for Diagnostics](https://biori-en.com/blood-based-molecular-profiling-reagents-diagnostics/): Caris’ NYSDOH application for a blood-based molecular profiling test highlights reagent and QC priorities for cfDNA extraction, NGS library prep, qPCR verification and OEM diagnostics. - [mRNA Manufacturing Raw Material Strategy After Capacity Shifts](https://biori-en.com/mrna-manufacturing-raw-material-strategy/): Recent mRNA manufacturing capacity shifts highlight why raw material strategy matters. This guide covers GMP-grade IVT enzymes, capping, DNase cleanup, QC and OEM planning for resilient RNA programs. - [Pharmacogenomic Testing Reagent Strategy for Clinical Diagnostics](https://biori-en.com/pharmacogenomic-testing-reagent-strategy/): Foundation Medicine’s new collaboration with Fulgent Genetics highlights rising clinical demand for pharmacogenomic testing. This guide explains reagent, QC and OEM considerations for diagnostic teams. - [cfDNA MRD Testing Reagent Strategy for Molecular Diagnostics](https://biori-en.com/cfdna-mrd-testing-reagent-qc-guide/): CareDx’s Naveris acquisition highlights rising interest in cfDNA MRD testing. This guide explains reagent, assay QC, sample prep and OEM considerations for molecular diagnostics teams. - [IVD Reagent Quality Documentation for OEM Programs](https://biori-en.com/ivd-reagent-quality-documentation-oem-programs/): New FDA Form 483 response guidance highlights documentation discipline. This guide explains what IVD reagent OEM teams should prepare for QC, CoA and traceability. - [Automated IVD Reagents for Respiratory Panels: QC and OEM Lessons](https://biori-en.com/automated-ivd-reagents-respiratory-panels-qc-oem/): Applied BioCode’s new FDA-cleared KingFisher extraction workflow for its respiratory panel highlights why automated IVD reagents need robust QC, OEM documentation and platform compatibility. - [NGS Sample Preparation for Molecular Diagnostics: Reagent Strategy and QC](https://biori-en.com/ngs-sample-preparation-molecular-diagnostics-reagent-qc/): A practical guide to NGS sample preparation for molecular diagnostics, covering input QC, library prep reagent selection, workflow consistency, and OEM considerations. - [Residual DNA Testing for Gene Therapy Manufacturing: A Practical QC Guide](https://biori-en.com/residual-dna-testing-gene-therapy/): A practical guide to residual DNA testing in gene therapy and biomanufacturing QC, covering method selection, sample prep, HCP/HCD risk, and supplier considerations. - [Meet Biori at ASGCT 2026 Boston,MA-Booth 1511](https://biori-en.com/biori-asgct-2026-booth-1511-hcd-hcp-universal-nuclease-custom-kits/): Meet Biori at ASGCT 2026, Booth No. 1511, to discuss HCD/HCP testing solutions, universal nuclease, and customized kit development services for CGT manufacturing and QC. - [Meet Biori at BIO KOREA 2026 — Visit Booth L13](https://biori-en.com/biori-bio-korea-2026-booth-l13/): Biori will attend BIO KOREA 2026 in Seoul from April 28–30. Visit us at Booth L13 to discuss IVD raw materials, PCR, NGS, IVT, OEM, and biopharma QC solutions. - [mRNA Bird Flu Vaccine IVT Enzymes and QC Guide](https://biori-en.com/mrna-bird-flu-vaccine-ivt-enzymes-qc-guide/): Explore the mRNA bird flu vaccine workflow, from GMP-grade IVT enzymes and capping to residual DNA and HCP testing for biopharma QC. - [Biori Biotech to Exhibit at Lab Indonesia 2026](https://biori-en.com/biori-biotech-to-exhibit-at-lab-indonesia-2026/): Biori Biotech will participate in Lab Indonesia 2026 at Booth 5D37, presenting innovative products and services for life science research, biotherapeutic analytical workflows, and molecular diagnostic product development. - [Enzyme-Based Library Preparation: The Genetic Scissors Driving the NGS Revolution](https://biori-en.com/enzyme-based-library-preparation-ngs/): Enzyme-based library preparation is reshaping next-generation sequencing by simplifying DNA fragmentation, improving automation compatibility, and reducing sample loss. This article explains how enzymatic fragmentation is driving the NGS revolution. - [qPCR Results Analysis: How to Interpret Amplification Curves, Melt Curves, and Ct Values](https://biori-en.com/qpcr-results-analysis/): This guide explains how to analyze qPCR results, including amplification curve interpretation, melt curve analysis, and Ct value meaning, to help improve data accuracy and assay reliability. - [12 Golden Rules for PCR Primer Design](https://biori-en.com/12-golden-rules-for-pcr-primer-design/): PCR primer design is one of the most important steps in developing a successful PCR assay. Well-designed primers directly affect specificity, amplification efficiency, sensitivity, and reproducibility. Poor primer design, on the other hand, can lead to nonspecific amplification, low yield, primer-dimer formation, or even complete reaction failure. - [Meet Biori at CACLP 2026 | Booth 4-1506](https://biori-en.com/biori-at-caclp-2026/): Biori will attend CACLP 2026 from March 21–23 in Xiamen, China. Visit us at Booth 4-1506 to explore our lyophilization solutions, contamination-proof LAMP raw materials, ultra-fast PCR raw materials, and fully premixed high-stability raw materials. We look forward to meeting you there. - [PCR vs qPCR vs RT-PCR vs RT-qPCR vs dPCR: Key Differences Explained](https://biori-en.com/pcr-vs-qpcr-vs-rtpcr-vs-rtqpcr-vs-dpcr/): In molecular biology and diagnostics, you’ll often see terms like PCR, qPCR (real-time PCR), RT-PCR, RT-qPCR, and dPCR (digital PCR). They sound similar, but they serve different purposes—especially in template type (DNA vs RNA) and whether results are qualitative, relative, or absolute. ## Pages - [Download](https://biori-en.com/download/): Download Molecular Diagnostics Brochure-2026 mRNA & IVT Brochure-2026 Quality Control (QC) Brochure-2026 NGS & Genomics Brochure-2026 Life Science Research Brochure-2026 - [NGS Selection](https://biori-en.com/product/ngs-selection/): Home - [QA/QC Product Selection](https://biori-en.com/qc-selection/): Home - [Life Science Research Selection](https://biori-en.com/life-science-research-selection/): Home - [Lyophilization Service for Molecular Diagnostics](https://biori-en.com/service/lyophilization-service-for-molecular-diagnostics/): Room-Temp Shipping - [OEM Service](https://biori-en.com/service/oem-service/): A flexible, end-to-end OEM offering—from wet formats to lyophilized beads, with specs, packaging, and documentation tailored to your needs. - [HCD/HCP Custom Assay Development Service](https://biori-en.com/service/hcd-hcp-custom-assay-development-service/): Two end-to-end customization tracks to match your process and regulatory needs. - [Service](https://biori-en.com/service/): End-to-end OEM/ODM support for molecular diagnostic enzymes and master mixes—from feasibility and formulation to pilot runs and scaled manufacturing. Designed for performance, manufacturability, and consistent lot-to-lot quality. - [Manufacturing & Quality](https://biori-en.com/manufacturing-quality/): Scalable Capacity - [Distributors](https://biori-en.com/distributors/): Distributor - [Molecular Diagnostics Selection](https://biori-en.com/molecular-diagnostics-selection/): Home - [Enquiry Cart](https://biori-en.com/enquiry-cart/): Upon submission, the inquiry details and product list will be automatically sent to your email address. Should you not receive the email, please check your spam folder or mail us to resend - [Product](https://biori-en.com/product/) - [About Us](https://biori-en.com/about-us/): Founded in 2012, Zhuhai Biori Biotechnology Co., Ltd. provides biological raw materials and reagent solutions for life science research, in vitro diagnostics (IVD), and biopharmaceutical applications. The company builds on established capabilities in protein engineering, recombinant expression, process development, and application-focused research to support both research and industrial customers worldwide. - [Contact Us](https://biori-en.com/get-in-touch/): Should you have any needs, please let us know, we will get back to you asap. - [Home](https://biori-en.com/): Custom Enzyme Engineering & OEM Manufacturing - [Privacy Policy](https://biori-en.com/privacy-policy/): An anonymized string created from your email address (also called a hash) may be provided to the Gravatar service to see if you are using it. The Gravatar service privacy policy is available here: https://automattic.com/privacy/. After approval of your comment, your profile picture is visible to the public in the context of your comment. - [Terms Of Services](https://biori-en.com/terms-of-services/): By accessing this Website at https://biori-en.com, you agree to be bound by these Website Terms and Conditions of Use and agree that you are responsible for compliance with any applicable local laws. If you disagree with any of these terms, you are prohibited from accessing this site. The materials contained in this Website are protected by copyright and trademark law. ## Products - [Bacteria DNA Detection Kit (qPCR), BP-QN99](https://biori-en.com/product/bacteria-dna-detection-kit-qpcr-bp-qn99/): Bacteria DNA Detection Kit (qPCR), BP-QN99, is a fluorescent probe qPCR kit for qualitative detection of bacterial DNA contamination in biological samples. The assay targets conserved bacterial 16S rRNA coding regions and includes an internal control to support reliable workflow monitoring. It is supplied as a 50-reaction kit for contamination testing in cells, cell and gene therapy samples, and other biological products. - [Fungi DNA Detection Kit (qPCR), BP-QN78](https://biori-en.com/product/fungi-dna-detection-kit-qpcr-bp-qn78/): Fungi DNA Detection Kit (qPCR), BP-QN78, is a fluorescent probe qPCR kit for qualitative detection of fungal DNA contamination in biological samples. The assay targets conserved fungal 18S rRNA coding regions and includes an internal control to help monitor the reaction workflow. It is supplied as a 50-reaction kit for contamination testing in cells, cell and gene therapy samples, and other biological products. - [E.coli Residual DNA Detection Kit (qPCR), BP-QN24](https://biori-en.com/product/e-coli-residual-dna-detection-kit-qpcr-bp-qn24/): E.coli Residual DNA Detection Kit (qPCR), BP-QN24, provides probe-based quantitative detection of E.coli residual DNA in intermediates, semi-finished products, and finished biological or pharmaceutical products. The kit uses fluorescent qPCR chemistry with an internal standard and calibrators to support residual host-cell DNA testing during bioprocess quality control. It is supplied as a 100 reactions/kit format for E.coli residual DNA quantification workflows. - [Mycoplasma DNA Detection Kit (qPCR), BP-QN22](https://biori-en.com/product/mycoplasma-dna-detection-kit-qpcr-bp-qn22/): Mycoplasma DNA Detection Kit (qPCR), BP-QN22, is a fluorescent probe qPCR kit for qualitative detection of Mycoplasma contamination in biological materials. The assay targets conserved Mycoplasma 16S rRNA regions and includes an internal control to support contamination testing in cell cultures, experimental animal secretions, animal sera, and related samples. It is supplied as a 50-reaction kit for research and manufacturing quality-control workflows. - [Bacteria & Fungi Nucleic Acid Extraction Kit (Magnetic Beads Method), BP-QN188](https://biori-en.com/product/bacteria-and-fungi-nucleic-acid-extraction-kit-magnetic-beads-method-bp-qn188/): Bacteria & Fungi Nucleic Acid Extraction Kit (Magnetic Beads Method), BP-QN188, extracts and purifies bacterial and fungal nucleic acids from biological-product samples for downstream PCR detection. The kit combines lysis chemistry with magnetic bead binding, washing, and elution to prepare microbial nucleic acids from complex sample matrices such as cell cultures, vaccines, and cell-therapy products. It is supplied as a 100-reaction BIORI kit for microbial nucleic acid preparation workflows. - [Nucleic Acid Extraction Kit II (Magnetic Beads Method), BP-QN11](https://biori-en.com/product/nucleic-acid-extraction-kit-ii-magnetic-beads-method-bp-qn11/): Nucleic Acid Extraction Kit II (Magnetic Beads Method), BP-QN11, is designed for extraction, enrichment, and purification of sample nucleic acids before PCR detection. The workflow uses guanidine salt lysis, magnetic bead adsorption, ethanol-containing wash steps, and high-temperature elution to generate PCR-ready eluates. It is supplied as a 100 reactions kit for laboratories that need a magnetic-bead extraction workflow for downstream nucleic acid testing. - [CHO Residual DNA Detection Kit (qPCR), BP-QN06](https://biori-en.com/product/cho-residual-dna-detection-kit-qpcr-bp-qn06/): CHO Residual DNA Detection Kit (qPCR), BP-QN06, provides probe-based quantitative detection of residual CHO host-cell DNA in intermediates, semi-finished products, and finished biological or pharmaceutical products. The kit uses fluorescent qPCR chemistry with an internal standard to monitor extraction and amplification performance, helping QC teams evaluate process-related DNA impurities. It is supplied as a 100 reactions/kit format and includes CHO qPCR mix, internal standard, negative control, and calibrators for residual DNA quantification workflows. - [Nucleic Acid Extraction Kit I (Magnetic Beads Method), BP-QN01-100](https://biori-en.com/product/nucleic-acid-extraction-kit-i-magnetic-beads-method-bp-qn01-100/): Nucleic Acid Extraction Kit I (Magnetic Beads Method) is a 100-reaction magnetic bead kit for nucleic acid extraction, enrichment, and purification before PCR detection. - [T7 RNA Polymerase ELISA Kit, BP-QP-T009](https://biori-en.com/product/t7-rna-polymerase-elisa-kit-bp-qp-t009/): T7 RNA Polymerase ELISA Kit, BP-QP-T009, is a 96 T/Kit double-antibody sandwich ELISA kit for quantifying T7 RNA Polymerase in research samples. The assay uses anti-T7 RNA Polymerase coated plate, detection antibody, T7 RNA Polymerase stock solution, sample diluent, wash buffer, TMB substrate, and stop solution for colorimetric measurement. The manual states a 1-64 ng/mL standard curve range, a 0.1 ng/mL detection limit, intra-batch CV below 10%, inter-batch CV below 15%, and recovery between 80% and 120%. - [RNase Inhibitor ELISA Kit, BP-QP-R014](https://biori-en.com/product/rnase-inhibitor-elisa-kit-bp-qp-r014/): RNase Inhibitor ELISA Kit, BP-QP-R014, is a 96 T/Kit double-antibody sandwich ELISA kit for quantifying RNase Inhibitor in research and manufacturing samples. The assay uses anti-RNase Inhibitor coated plate, detection antibody, RNase Inhibitor standard, sample diluent, wash buffer, TMB substrate, and stop solution for colorimetric measurement. The manual states a 0.23-57 ng/mL standard curve range, a 0.23 ng/mL quantitation limit, intra-batch CV below 10%, inter-batch CV below 15%, and recovery between 75% and 125%. - [DNase I ELISA Kit, BP-QP-D013](https://biori-en.com/product/dnase-i-elisa-kit-bp-qp-d013/): DNase I ELISA Kit, BP-QP-D013, is a 96 T/Kit double-antibody sandwich ELISA kit for quantifying DNase I in research and manufacturing samples. The assay uses anti-DNase I coated microplate strips, anti-DNase I detection antibody, DNase I standard, sample diluent, wash buffer, TMB substrate, and stop solution. The manual states a 1-64 ng/mL standard curve range, a 0.1 ng/mL quantitation limit, intra-batch CV below 10%, inter-batch CV below 15%, and recovery between 80% and 120%. - [CHO Host Cell Protein (HCP) ELISA Kit-V2, BP-QP-C029](https://biori-en.com/product/cho-host-cell-protein-hcp-elisa-kit-v2-bp-qp-c029/): CHO Host Cell Protein (HCP) ELISA Kit-V2, BP-QP-C029, is a 96 T/Kit double-antibody sandwich ELISA kit for quantifying CHO host cell protein in bioproducts expressed and amplified using CHO. The assay uses anti-CHO HCP coated microplate strips, CHO HCP detection antibody, standards, sample diluent, wash buffer, TMB substrate, and stop solution for colorimetric HCP measurement. The manual states a 1.5-200 ng/mL standard curve range, a 1.5 ng/mL quantitation limit, intra-batch CV below 10%, and inter-batch CV below 15%. - [BSA (Bovine Serum Albumin) ELISA Kit, BP-QP-B024](https://biori-en.com/product/bsa-bovine-serum-albumin-elisa-kit-bp-qp-b024/): BSA (Bovine Serum Albumin) ELISA Kit, BP-QP-B024, is a 96 T/Kit double-antibody sandwich ELISA kit for quantifying BSA in research samples. The assay uses anti-BSA coated microplate strips, anti-BSA enzyme-conjugated antibody, BSA standards, wash buffer, TMB substrate, and stop solution for colorimetric measurement. The manual states a 1.5-50 ng/mL standard curve range, a 1.5 ng/mL quantitation limit, intra-batch CV below 10%, inter-batch CV below 15%, and no observed cross reaction with 10 mg/mL HSA. - [EcoR I GMP-grade, GMP-BP-E16](https://biori-en.com/product/ecor-i-gmp-grade-gmp-bp-e16/): EcoR I GMP-grade is a GMP-grade restriction endonuclease for precise DNA digestion, sticky-end generation, and linearized DNA template preparation in molecular biology and GMP-oriented workflows. - [BspQ I GMP-grade, GMP-BP-E09](https://biori-en.com/product/bspq-i-gmp-grade-gmp-bp-e09/): BspQ I GMP-grade is a GMP-grade type IIS restriction endonuclease for precise DNA digestion, generation of sticky ends, and preparation of linearized DNA fragments in molecular biology and mRNA workflow applications. - [Bsa I GMP-grade, GMP-BP-E10](https://biori-en.com/product/bsa-i-gmp-grade-gmp-bp-e10/): GMP-grade Bsa I type IIS restriction endonuclease for precise DNA digestion, sticky-end generation, and linearized fragment preparation in molecular biology and biopharma workflows. - [T7 RNA Polymerase 3.0 GMP-grade (low dsRNA), GMP-BP-E11](https://biori-en.com/product/t7-rna-polymerase-3-0-gmp-grade-low-dsrna-gmp-bp-e11/): GMP-grade T7 RNA Polymerase 3.0 for in vitro transcription with reduced dsRNA by-product formation, high catalytic activity, and reliable performance for mRNA synthesis workflows. - [RNase R GMP-grade for Linear RNA Removal and circRNA Enrichment, GMP-BP-E08](https://biori-en.com/product/rnase-r-gmp-grade-for-linear-rna-removal-and-circrna-enrichment-gmp-bp-e08/): RNase R GMP-grade is a recombinant magnesium-dependent 3′ to 5′ exoribonuclease for selective linear RNA removal, circRNA enrichment, lariat RNA analysis, and alternative splicing studies. Supplied at 20 U/μL with dedicated buffer systems, it is designed for high-standard RNA research, RNA process development, and GMP-oriented biopharma workflows requiring robust enzyme performance and stringent quality control. - [mRNA Cap 2′-O-Methyltransferase GMP-grade, GMP-BP-E06](https://biori-en.com/product/mrna-cap-2-o-methyltransferase-gmp-grade-gmp-bp-e06/): mRNA Cap 2'-O-Methyltransferase GMP-grade (2'-O-Methyltransferase, 2OM) is a recombinant vaccinia virus DNA-encoded methyltransferase expressed in E. coli. It catalyzes the conversion of the Cap0 structure to the Cap1 structure by transferring a methyl group to the 2'-O position of the first nucleotide adjacent to the RNA 5' cap, using S-adenosylmethionine (SAM) as the methyl donor. This enzymatic modification generates Cap1-capped mRNA, which can further reduce the intrinsic immunogenicity of mRNA and improve expression of the encoded protein after transfection. Supplied with 10× Capping Buffer and SAM, this GMP-grade enzyme is suitable for high-quality mRNA capping workflows in research and bioprocess development. Biori mRNA Cap 2'-O-Methyltransferase GMP-grade is designed for stepwise and one-step capping systems requiring reliable Cap0-to-Cap1 conversion and strong quality attributes for advanced mRNA applications. - [Aci I, BR1G102](https://biori-en.com/product/aci-i-br1g102/): Aci I is a Type IIP restriction endonuclease expressed in Escherichia coli carrying the Aci I gene cloned from Arthrobacter citreus. It specifically recognizes and cleaves the sequence 5'...C↓CGC...3' / 3'...GGC↑G...5'. Supplied with 10× Cut-Buffer, Aci I is suitable for routine DNA digestion workflows involving genomic DNA, plasmid DNA, and PCR products. This enzyme is optimized for incubation at 37°C and provides reliable restriction digestion performance for molecular biology research applications. Biori Aci I is designed for research use in restriction enzyme digestion workflows requiring sequence-specific DNA cleavage and convenient heat inactivation after digestion. - [FEN1, BR1G101](https://biori-en.com/product/fen1-br1g101/): FEN1 is a thermostable nuclease derived from the FEN1 gene of Thermococcus 9°N. It specifically recognizes and cleaves 5′ flap DNA structures in branched double-stranded DNA substrates, generating ligatable 5′-phosphate ends for downstream DNA ligation. The cleavage products generated by FEN1 can be efficiently ligated by DNA ligase to form double-stranded DNA, making this enzyme useful for structure-specific DNA processing workflows. In vivo, FEN1 plays an essential role in Okazaki fragment maturation and also participates in base excision repair. Supplied with a dedicated 10 × reaction buffer, Biori FEN1 is suitable for research applications involving 5′ flap DNA cleavage and structure-specific DNA analysis. - [SeamLess Cloning Master Mix, BR1C101](https://biori-en.com/product/seamless-cloning-master-mix-br1c101/): SeamLess Cloning Master Mix is a simple, rapid, and efficient universal seamless cloning reagent developed by Biori. It enables rapid directional cloning of insert fragments into any site of virtually any vector. Designed for high cloning efficiency, this master mix delivers high colony numbers and high positive rates in homologous recombination-based assembly workflows. It supports the assembly of up to 15 insert fragments in a single reaction, making it suitable for both routine cloning and more complex construct design. SeamLess Cloning Master Mix is ideal for rapid cloning, high-throughput cloning, seamless cloning, and DNA site-directed mutagenesis workflows in molecular biology research. - [Neoscript Multi One-Step RT-qPCR Master Mix with UNG, M8254](https://biori-en.com/product/neoscript-multi-one-step-rt-qpcr-master-mix-with-ung-m8254/): Neoscript Multi One-Step RT-qPCR Master Mix with UNG is a dedicated reagent for one-step qualitative and quantitative RT-qPCR using the probe method and is suitable for multiplex amplification. The entire reaction is performed sequentially in a single tube, avoiding tube opening and effectively reducing contamination. The product contains Neoscript RTase, a thermostable reverse transcriptase with reduced RNase H activity, and a genetically engineered hot-start Taq DNA polymerase, providing higher reverse transcription and PCR amplification efficiency and making it suitable for highly sensitive amplification of low-concentration RNA templates. The reagent uses an optimized qPCR buffer system together with a UNG/dUTP anti-contamination system, enabling good standard curves over a broad dynamic range for accurate quantification while effectively preventing false-positive amplification caused by residual PCR products or aerosol contamination. - [AcuGenix™ FastAmpli qPCR Master Mix with UNG-V4, M2181-4](https://biori-en.com/product/acugenix-fastampli-qpcr-master-mix-with-ung-v4-m2181-4/): AcuGenix™ FastAmpli qPCR Master Mix with UNG-V4 is a dedicated reagent for qualitative and quantitative real-time PCR using probe-based chemistry. It contains a genetically engineered DNA polymerase optimized for rapid amplification, allowing the entire PCR reaction to be completed within 30 min. The buffer system has been optimized for multiplex amplification. This reagent combines an inhibitor-tolerant amplification enzyme, UNG, and an optimized buffer system containing dUTP, enabling robust amplification of target genes in inhibitor-containing samples while effectively reducing false-positive results caused by carryover PCR products and aerosol contamination. It is compatible with most real-time PCR instruments from Applied Biosystems, Eppendorf, Bio-Rad, and Roche. - [Vaccinia Capping Enzyme GMP-grade, GMP-BP-E05](https://biori-en.com/product/vaccinia-capping-enzyme-gmp-grade/): Mature mRNA in eukaryotes carries a unique 5′ cap structure (m⁷GpppN), known as the methylguanosine cap, which is essential for translation initiation, protection from RNase degradation, and enhancement of mRNA stability during splicing and nuclear export. Vaccinia Capping Enzyme GMP-grade (VCE), developed in-house by Biori Biotech, integrates the three enzymatic activities required for capping: RNA triphosphatase, guanylyltransferase, and guanine-N7-methyltransferase. In the presence of S-adenosylmethionine (SAM) as a methyl donor and guanosine triphosphate (GTP), VCE directly and efficiently adds an m⁷G cap to the 5′ end of mRNA in the correct orientation, producing Cap0-capped RNA with up to 100% capping efficiency in a single reaction. - [DNase I GMP-grade, GMP-BP-E04](https://biori-en.com/product/dnase-i-gmp-grade/): DNase I GMP-grade (Deoxyribonuclease I) is an endonuclease that efficiently digests single-stranded and double-stranded DNA. DNase I requires Ca²⁺ for activity and is activated by Mg²⁺ or Mn²⁺. In the presence of Mg²⁺, DNase I cleaves DNA at random sites; in the presence of Mn²⁺, the enzyme cleaves both strands of double-stranded DNA at approximately the same position, generating blunt-ended fragments or fragments with 1–2 nucleotide overhangs. DNase I GMP-grade exhibits optimal endonuclease activity at pH 7–8 and is suitable for removing single-stranded or double-stranded DNA from RNA preparations and other downstream workflows. - [Pyrophosphatase, Inorganic GMP-grade, GMP-BP-E03](https://biori-en.com/product/inorganic-pyrophosphatase-gmp-grade/): Inorganic Pyrophosphatase GMP-grade is a recombinant inorganic pyrophosphatase expressed and purified from E. coli. It catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to orthophosphate (Pi). During in vitro transcription, the enzyme hydrolyzes PPi generated as a by-product of the reaction, relieving its inhibitory effect on the transcription reaction and shifting the equilibrium toward RNA product formation. Addition of this enzyme in in vitro transcription reactions significantly improves RNA yield and supports efficient RNA production workflows. - [RNase Inhibitor GMP-grade, GMP-BP-E02](https://biori-en.com/product/rnase-inhibitor-gmp-grade-gmp/): RNase Inhibitor GMP-grade is a recombinant RNase inhibitor produced through recombinant expression and multi-step purification. It inhibits RNase A, RNase B, and RNase C activity through high-affinity non-covalent 1:1 binding, while having no effect on RNase H or S1 Nuclease activity. This product does not inhibit phage RNA polymerases (SP6, T7, or T3), Taq DNA polymerase, AMV reverse transcriptase, or M-MLV reverse transcriptase. RNase Inhibitor GMP-grade exhibits enhanced oxidative stability and maintains activity under low DTT conditions. It is suitable for excluding RNase contamination in mRNA in vitro transcription, storage, purification, reverse transcription, qPCR, and other RNA-related applications requiring intact RNA. - [T7 RNA Polymerase GMP-grade, GMP-BP-E01](https://biori-en.com/product/t7-rna-polymerase-gmp-grade-gmp-bp-e01/): T7 RNA Polymerase GMP-grade is an in-house developed and multi-step purified recombinant T7 RNA polymerase manufactured by Biori Biotech to GMP standards. It exhibits high transcriptional catalytic activity across diverse template types and nucleotide substrates. This enzyme specifically recognizes the T7 promoter sequence (5′-TAATACGACTCACTATAG-3′) and initiates transcription from the G residue at this site, converting downstream DNA sequences into single-stranded RNA. Using natural or modified nucleotides as substrates, and under appropriate reaction buffer conditions, T7 RNA Polymerase GMP-grade enables efficient synthesis of large quantities of RNA from DNA templates. A single 20 µL in vitro transcription reaction can yield more than 200 µg of RNA. - [AcuGenix™ Hot Start Taq DNA Polymerase (Glycerol-Free), FE03](https://biori-en.com/product/acugenix-hot-start-taq-dna-polymerase-glycerol-free-fe03/): AcuGenix™ Hot Start Taq DNA Polymerase (Glycerol-Free) is a chemically modified hot-start Taq DNA polymerase optimized and formulated specifically for the special requirements of freeze-dried reagents. It completely blocks Taq enzyme activity at room temperature and effectively suppresses non-specific amplification caused by primer annealing or primer dimerization at low temperatures, thereby improving the specificity and sensitivity of PCR reactions. It can also work in a “time release” mode to gradually release enzyme activity during PCR cycling, further enhancing amplification specificity and sensitivity for low-copy templates. In fluorescent PCR applications, it offers high sensitivity, high fluorescence intensity, and high specificity. - [E.coli Host Cell Protein (HCP) ELISA Kit-V2, BP-QP-E028](https://biori-en.com/product/e-coli-host-cell-protein-hcp-elisa-kit-v2-bp-qp-e028/): E.coli Host Cell Protein (HCP) ELISA Kit-V2, BP-QP-E028, is a 96 T/Kit double-antibody sandwich ELISA kit for quantifying E.coli host cell protein in bioproducts expressed and amplified using E.coli. The assay uses anti-E.coli HCP coated plate, E.coli HCP detection antibody, standards, sample diluent, wash buffer, TMB substrate, and stop solution for colorimetric HCP measurement. The manual states a 2-100 ng/mL standard curve range, a 1.5 ng/mL quantitation limit, intra-batch CV below 10%, inter-batch CV below 15%, and recovery between 80% and 120%. - [Robustart Fast Taq DNA Polymerase-V3,E40](https://biori-en.com/product/robustart-fast-taq-dna-polymerase-v3/): Robustart Fast Taq DNA Polymerase-V3 is a hot-start rapid amplification Taq enzyme optimized for freeze-dried reagent systems. It effectively suppresses non-specific amplification caused by primer misannealing or primer-dimer formation during PCR setup and amplification, delivering high specificity and improved performance for low-concentration templates. With good system adaptability, it provides stable amplification results in different PCR reaction types and is suitable for multiplex PCR amplification. - [Robustart Fast Taq DNA Polymerase-V2, E20](https://biori-en.com/product/robustart-fast-taq-dna-polymerase-v2/): Robustart Fast Taq DNA Polymerase-V2 is a hot-start fast amplification DNA polymerase developed by Biori. The enzyme effectively suppresses non-specific amplification caused by primer misannealing or primer-dimer formation during PCR setup and amplification. This enzyme features rapid DNA amplification capability and improved tolerance to common PCR inhibitors, enabling reliable amplification even from low-concentration templates. Robustart Fast Taq DNA Polymerase-V2 is suitable for conventional PCR, rapid PCR assays, multiplex PCR, and nucleic acid detection applications. - [Robustart Taq DNA Polymerase, E16](https://biori-en.com/product/robustart-taq-dna-polymerase/): Robustart Taq DNA Polymerase is a hot-start PCR enzyme developed for high-specificity DNA amplification. The enzyme effectively suppresses non-specific reactions caused by primer misannealing or primer-dimer formation during PCR setup and amplification. It provides improved amplification efficiency for low-concentration DNA templates and demonstrates strong system adaptability across various PCR assays. Robustart Taq DNA Polymerase is suitable for conventional PCR, quantitative PCR, and multiplex PCR applications, delivering reliable and stable amplification performance. - [SuperStart Taq Plus DNA Polymerase, E07](https://biori-en.com/product/superstart-taq-plus-dna-polymerase/): SuperStart Taq Plus DNA Polymerase is a novel hot-start DNA polymerase developed by Biori. The enzyme utilizes antibody-based hot-start modification to effectively suppress non-specific amplification caused by primer misannealing or primer-dimer formation during PCR setup and amplification. This enzyme demonstrates excellent performance in multiplex PCR applications and provides optimized amplification efficiency for extremely low-concentration DNA templates. It enables higher product yields, improved specificity, and stable amplification results, making it suitable for both conventional PCR and quantitative PCR assays. - [AcuGenix™ Hot Start Taq DNA Polymerase, E03](https://biori-en.com/product/acugenix-hot-start-taq-dna-polymerase/): AcuGenix™ Hot Start Taq DNA Polymerase is a chemically modified hot-start enzyme designed to prevent non-specific amplification and primer-dimer formation. 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