Neoscript RT Premix-UNG (Probe qRT-PCR) is a specialized reagent for qualitative and quantitative one-step fluorescent quantitative PCR using probes. This product contains customized high-temperature reverse transcriptase with RNase H activity removed and a new type of hot-start enzyme, which has higher efficiency in both reverse transcription and PCR amplification, making it suitable for highly sensitive amplification of low-concentration RNA templates. The optimized qPCR-specific buffer and UNG/dUTP contamination prevention system used in this reagent allows for accurate quantification within a wide range of concentrations, preventing false-positive amplifications caused by residual PCR products or aerosol contamination.
Product Specification:
System Volume: 50μl
Lyophilization: Yes
Reagent Composition
- 25×Neoscript RTase/UNG Mix
- 5×Neoscript RT Premix Buffer (dUTP)
- 4×lyoprotectant (optional)
Recommended Applications:
- COVID-19 detection.
- Respiratory diseases detection.
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