Pre-treatment Kit for mRNA Poly(A) Tail Distribution Detection

Detection of mRNA poly(A) tail distribution is an important step in molecular biology research, contributing to a deeper understanding of mRNA stability and translational regulatory mechanisms. The poly(A) tail, as the 3' end structure of mRNA, composed of multiple adenosine (A) residues, is crucial for mRNA function, and its length and distribution are essential factors.

Product Features:

Efficient purification: The kit utilizes optimized purification techniques to rapidly and effectively isolate high-quality mRNA poly(A) tails from complex samples, providing reliable support for subsequent detection.

Easy operation: Simplifies traditional cumbersome operational steps, enabling researchers to easily handle and quickly complete the pre-treatment process, saving valuable time and effort.

High sensitivity and specificity: The kit exhibits excellent sensitivity and specificity, accurately capturing low-abundance mRNA poly(A) tails, providing precise data support for research.

Safe and reliable: Strict control of product quality ensures the stability and reliability of the kit, making experimental results more trustworthy and reproducible.

After RNase T1 digestion, there are still many impurities in the kit. However, after purification and recovery, the purity of the Poly(A) tail bands is significantly improved. This improvement is also reflected in the TIC (Total Ion Chromatogram), where the target peak shows a higher response value and appears earlier in the retention time.

The ion flow in the target peak exhibits a trend of normal distribution. Through deconvolution and spectrum interpretation, a standard normal distribution or near-normal distribution of Poly(A) can be obtained.

• BP-11-10 说明书

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