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Product Catalog
Inorganic Pyrophosphatase GMP-grade is a recombinant inorganic pyrophosphatase expressed and purified from E. coli. It catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to orthophosphate (Pi).
During in vitro transcription, the enzyme hydrolyzes PPi generated as a by-product of the reaction, relieving its inhibitory effect on the transcription reaction and shifting the equilibrium toward RNA product formation.
Addition of this enzyme in in vitro transcription reactions significantly improves RNA yield and supports efficient RNA production workflows.
Pyrophosphatase, Inorganic GMP-grade, GMP-BP-E03
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Features
- Recombinant inorganic pyrophosphatase expressed and purified from E. coli
- Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to orthophosphate (Pi)
- Relieves the inhibitory effect of PPi generated during in vitro transcription
- Shifts the reaction equilibrium toward RNA product formation
- Significantly improves RNA yield in in vitro transcription reactions
- Active in a wide range of reaction buffers and can generally be added directly to the reaction system
Application
- Enhancing RNA yield in in vitro transcription reactions
- Promoting DNA and protein synthesis
- Catalyzing the hydrolysis of inorganic pyrophosphate to orthophosphate
Components
| No. | Component | Catalog No. | Quantity | Volume |
|---|---|---|---|---|
| 1 | Inorganic Pyrophosphatase GMP-grade (0.1 U/µL) | GMP-BP-E03-10 | 10 U | 100 µL |
| 2 | Inorganic Pyrophosphatase GMP-grade (0.1 U/µL) | GMP-BP-E03-100 | 100 U | 1 mL |
| 3 | Inorganic Pyrophosphatase GMP-grade (0.1 U/µL) | GMP-BP-E03-1K | 1,000 U | 10 mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at −20 ± 5 °C. |
| Handling | Mix gently before use. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% (v/v) Triton X-100, 50% (v/v) glycerol |
Data
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