IVT Enzymes
Browse BIORI's product-specific GMP-grade enzyme catalog for IVT and related mRNA workflows, including T7 RNA polymerases, RNase inhibitor, inorganic pyrophosphatase, DNase I, Vaccinia capping enzyme, Cap 2'-O-Methyltransferase, RNase R, and selected restriction endonucleases.
One recommended product per use case
Start from what you need to do. Each row names the product we would pick first and the alternatives in the same group; the full list is below.
| Use case | Recommended product | Why this one | Alternatives |
|---|---|---|---|
| Plasmid digestion for linearized DNA fragment preparation | Bsa I GMP-grade GMP-BP-E10 Data | GMP-grade Bsa I restriction enzyme for mRNA and IVT template preparation. | GMP-BP-E09, GMP-BP-E16 |
| Preparation of DNA-free RNA samples for downstream RNA… | DNase I GMP-grade GMP-BP-E04 Data | DNase I enzyme for DNA digestion in RNA workflows. | — |
| Conversion of Cap0 RNA to Cap1 RNA in a stepwise capping… | mRNA Cap 2'-O-Methyltransferase GMP-grade GMP-BP-E06 Data | Methyltransferase for converting Cap0 RNA to Cap1 RNA. | — |
| Used to hydrolyze the inorganic pyrophosphate generated… | Pyrophosphatase, Inorganic GMP-grade GMP-BP-E03 Data | Recombinant inorganic pyrophosphatase for IVT workflows. | — |
| During IVT, it is used to inhibit RNases in the… | RNase Inhibitor GMP-grade GMP-BP-E02 Data | Recombinant RNase inhibitor for protecting RNA workflows. | — |
| (1) Enrichment and purification of circular RNA by… | RNase R GMP-grade GMP-BP-E08 Data | RNase R enzyme for digesting linear RNA. | — |
| In vitro transcription and mRNA process development… | T7 RNA Polymerase 3.0 GMP-grade (low dsRNA) GMP-BP-E11 Data | Low-dsRNA T7 RNA polymerase for IVT mRNA synthesis. | GMP-BP-E01 |
| Cap 0 capping of mRNA for in vitro or in vivo translation | Vaccinia Capping Enzyme GMP-grade GMP-BP-E05 Data | Enzyme for Cap0 mRNA capping workflows. | — |
11 products
Bsa I GMP-grade
GMP-grade Bsa I restriction enzyme for mRNA and IVT template preparation.
BspQ I GMP-grade
GMP-grade BspQ I restriction enzyme for mRNA and IVT template preparation.
EcoR I GMP-grade
GMP-grade EcoR I restriction enzyme for mRNA and IVT template preparation.
mRNA Cap 2'-O-Methyltransferase GMP-grade
Methyltransferase for converting Cap0 RNA to Cap1 RNA.
Pyrophosphatase, Inorganic GMP-grade
Recombinant inorganic pyrophosphatase for IVT workflows.
T7 RNA Polymerase 3.0 GMP-grade (low dsRNA)
Low-dsRNA T7 RNA polymerase for IVT mRNA synthesis.
Documents
Manuals, COA and SDS are linked on each product row and on the product page.
Custom and OEM
Bulk fills, custom formulations and pilot evaluation are quoted per project.
Talk to an expert →Frequently asked questions
Which products are in the IVT Enzymes category?
What documents are provided?
Can I order bulk packs or a custom formulation?
Need help choosing, or a custom format?
Send your workflow and requirements. We reply with a proposal and evaluation samples.
