mRNA Cap 2′-O-Methyltransferase GMP-grade, GMP-BP-E06

mRNA Cap 2′-O-Methyltransferase GMP-grade (2′-O-Methyltransferase, 2OM) is a recombinant vaccinia virus DNA-encoded methyltransferase expressed in E. coli. It catalyzes the conversion of the Cap0 structure to the Cap1 structure by transferring a methyl group to the 2′-O position of the first nucleotide adjacent to the RNA 5′ cap, using S-adenosylmethionine (SAM) as the methyl donor.

This enzymatic modification generates Cap1-capped mRNA, which can further reduce the intrinsic immunogenicity of mRNA and improve expression of the encoded protein after transfection. Supplied with 10× Capping Buffer and SAM, this GMP-grade enzyme is suitable for high-quality mRNA capping workflows in research and bioprocess development.

Biori mRNA Cap 2′-O-Methyltransferase GMP-grade is designed for stepwise and one-step capping systems requiring reliable Cap0-to-Cap1 conversion and strong quality attributes for advanced mRNA applications.