mRNA Cap 2′-O-Methyltransferase GMP-grade (2′-O-Methyltransferase, 2OM) is a recombinant vaccinia virus DNA-encoded methyltransferase expressed in E. coli. It catalyzes the conversion of the Cap0 structure to the Cap1 structure by transferring a methyl group to the 2′-O position of the first nucleotide adjacent to the RNA 5′ cap, using S-adenosylmethionine (SAM) as the methyl donor.
This enzymatic modification generates Cap1-capped mRNA, which can further reduce the intrinsic immunogenicity of mRNA and improve expression of the encoded protein after transfection. Supplied with 10× Capping Buffer and SAM, this GMP-grade enzyme is suitable for high-quality mRNA capping workflows in research and bioprocess development.
Biori mRNA Cap 2′-O-Methyltransferase GMP-grade is designed for stepwise and one-step capping systems requiring reliable Cap0-to-Cap1 conversion and strong quality attributes for advanced mRNA applications.
mRNA Cap 2′-O-Methyltransferase GMP-grade, GMP-BP-E06
Features
- Recombinant vaccinia virus DNA-encoded 2′-O-methyltransferase expressed in E. coli.
- Catalyzes efficient conversion of Cap0 RNA to Cap1-capped mRNA using SAM as the methyl donor.
- Helps reduce the intrinsic immunogenicity of mRNA and improve expression of the encoded protein after transfection.
- Supplied at 50 U/µL with 10× Capping Buffer and SAM for convenient reaction setup.
- Compatible with both stepwise capping and one-step capping workflows.
- Protein purity ≥ 95%, residual host DNA ≤ 100 pg/mg, residual host cell protein < 50 ppm, and bacterial endotoxin < 5 EU/mL.
- Free of exogenous DNase, RNase, exonuclease, and endonuclease activities.
- Pathogens (HBV/HCV/HIV) and mycoplasma: not detected.
Application
- Cap0-to-Cap1 conversion in mRNA capping workflows
- Post-transcriptional enzymatic mRNA cap maturation
- Stepwise capping systems
- One-step capping systems used with Vaccinia Capping Enzyme GMP-grade
- mRNA preparation workflows requiring reduced intrinsic immunogenicity
- mRNA applications requiring improved protein expression after transfection
Components
| Component | GMP-BP-E06-5K | GMP-BP-E06-50K | GMP-BP-E06-500K |
|---|---|---|---|
| Pack Size | 5 KU | 50 KU | 500 KU |
| mRNA Cap 2′-O-Methyltransferase GMP-grade (50 U/µL) | 0.1 mL | 1 mL | 10 mL |
| 10× Capping Buffer | 0.2 mL | 2 mL | 20 mL |
| SAM (32 mM) | 20 µL | 0.2 mL | 2 mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20 ± 5℃. |
| Handling | Mix thoroughly before use and avoid repeated freeze-thaw cycles. |
| SAM Handling | SAM is inherently unstable and should be thawed and dispensed on ice throughout the procedure. |
Data
Coming Soon…
