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Product Catalog
GMP-grade T7 RNA Polymerase 3.0 for in vitro transcription with reduced dsRNA by-product formation, high catalytic activity, and reliable performance for mRNA synthesis workflows.
T7 RNA Polymerase 3.0 GMP-grade (low dsRNA), GMP-BP-E11
USD
$0.00
GMP-BP-E11
Features
- Reduced dsRNA by-product generation compared with conventional T7 RNA polymerase
- High transcriptional catalytic activity across different DNA templates and nucleic acid types
- Precise recognition of the T7 promoter sequence (5′-TAATACGACTCACTATAG-3′)
- Supports RNA synthesis with natural or modified nucleotide substrates
- GMP-grade manufacturing with multi-step purification and stringent quality control
- Free of exogenous DNase, RNase, exonuclease, and endonuclease activity
- Low residual host-cell DNA, low endotoxin, and high protein purity for demanding IVT workflows
Application
- In vitro transcription (IVT) for mRNA synthesis and process development
- Preparation of RNA from DNA templates containing the T7 promoter
- Workflows requiring reduced dsRNA by-product formation to support downstream translational performance and lower innate immune activation risk
- Research, preclinical, and manufacturing-oriented therapeutic RNA development
- Use with natural or modified nucleotides in advanced RNA production workflows
Components
| No. | Component | Catalog No. | Quantity | Volume |
|---|---|---|---|---|
| 1 | T7 RNA Polymerase 3.0 GMP-grade (low dsRNA) | GMP-BP-E11-5K | 5 KU | 100 μL |
| 2 | T7 RNA Polymerase 3.0 GMP-grade (low dsRNA) | GMP-BP-E11-50K | 50 KU | 1 mL |
| 3 | T7 RNA Polymerase 3.0 GMP-grade (low dsRNA) | GMP-BP-E11-500K | 500 KU | 10 mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20 ± 5 °C. |
| Handling | Mix thoroughly before use and avoid repeated freeze-thaw cycles. |
| RNase precautions | Use RNase-free consumables and wear gloves throughout the workflow. |
| Storage Buffer | 50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% (v/v) Triton X-100, 50% (v/v) glycerol, pH 7.9 at 25 °C. |
Data
Coming Soon…
