Bst 3.0

Bst 3.0 is derived from Bacillus stearothermophilus DNA Polymerase. Through genetic engineering technology, it retains 5'→3' DNA polymerase activity and removes 5'→3' exonuclease activity. It has strand displacement ability and can be used for DNA strand displacement reaction and LAMP (Loop-mediated isothermal amplification) amplification. Bst 3.0 DNA polymerase has been genetically modified to have higher sensitivity. Compared with wild-type Bst DNA polymerase and Bst large fragment, this enzyme can effectively improve the anti-interference performance of inhibitors, amplification speed, yield, etc. Bst 3.0 DNA polymerase can use dUTP to build LAMP anti-contamination system.

Compositions：

1. 8 U/µL Bst 3.0 

2. 10×LAMP Basic Buffer (Mg²+ free)（Optional） 

3. 250 mM MgSO₄（Optional） 

*10×LAMP Basic Buffer (Mg²+ free) does not include dNTP or Mg²+，Please add dNTPs and MgSO₄ when preparing reaction system. 

Features：

1.  Suitable for LAMP amplification.   

2.  Strong anti-inhibition ability, high impurity tolerance with high sample compatibility.

Recommended Applications: 

1. LAMP Isothermol amplification.

2. Establishment of anti-contamination system.