ISO 13485 & 9001 certified
Enzymes, master mixes and lyoprotectants side by side: the features that set each product apart, and the catalog number of every version, from standard to glycerol-free, low-residual DNA and lyo-ready.
For fast cycling or crude samples, choose Robustart Fast Taq-V2 E20 or -V3 E40: 30 s activation and direct PCR from blood and swabs. Hot Start Taq E03 is chemically modified and needs 5–10 min at 95 °C. Robustart Tth E17 also has reverse-transcriptase activity in the presence of Mn²⁺, for single-enzyme RT-PCR.
| Key differences | Catalog no. by version | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Product | Hot start | Hot-start activation | Fast PCR | Inhibitor tolerance / direct PCR | Reverse transcriptase activity | Standard | Glycerol-free | Low-residual DNA | Glycerol-free + low-residual DNA |
| Hot Start TaqE03 | Chemical modification | 95°C 5–10 min | ✕No | ✕No | ✕No | E03 | FE03 | E03-NA | FE03-NA |
| Robustart TaqE16 | Antibody | 95°C 1–5 min | ✕No | ✕No | ✕No | E16 | FE16 | E16-NA | FE16-NA |
| Robustart Fast Taq-V2E20 | Antibody | 95°C 30 s | Yes | Blood, swabs; direct PCR | ✕No | E20 | FE20 | E20-NA | FE20-NA |
| Robustart Fast Taq-V3E40 | Antibody | 95°C 30 s | Yes | Blood, swabs, phenol/alcohol residues; direct PCR | ✕No | E40 | FE40 | E40-NA | FE40-NA |
| Robustart TthE17 | Antibody | 95°C 1–5 min | ✕No | Various inhibitors | YesWith Mn²⁺ | E17 | FE17 | –Not applicable or no data | –Not applicable or no data |
Hot-start versions E103, E105 allow room-temperature setup. The V3 enzymes E104, E105 add inhibitor tolerance and dUTP tolerance (partial for E105) for UNG carry-over control.
| Key differences | Catalog no. by version | ||||
|---|---|---|---|---|---|
| Product | Hot start (room-temp setup) | Inhibitor tolerant | dUTP tolerant (UNG-compatible) | Standard | Glycerol-free |
| Bst-V2E102 | ✕No | ✕No | ✕No | E102 | FE102 |
| Bst Hot Start-V2E103 | YesReversible modification | ✕No | ✕No | E103 | FE103 |
| Bst-V3E104 | ✕No | Yes | Yes | E104 | FE104 |
| Bst Hot Start-V3E105 | YesReversible modification | Yes | YesPartial dUTP tolerance | E105 | FE105 |
Neoscript RT-V2 E42 works at 42–55 °C. The hot-start version E43 uses a thermal-activation modification and runs at 50–55 °C. Both are inhibitor tolerant.
| Key differences | Catalog no. by version | ||||||
|---|---|---|---|---|---|---|---|
| Product | Hot start | RT temperature | Inhibitor tolerant | Standard | Glycerol-free | Low-residual DNA | Glycerol-free + low-residual DNA |
| Neoscript RT-V2E42 | ✕No | 42–55 °C | Yes | E42 | FE42 | E42-NA | FE42-NA |
| Neoscript Hot Start RT-V2E43 | YesThermal-activation modification | 50–55 °C | Yes | E43 | FE43 | E43-NA | FE43-NA |
UNG/UDG E01 digests at 50 °C and is inactivated at 95 °C, so it suits PCR. Heat-labile UNG-V2 E04-2 digests at 20–37 °C and is inactivated at 50 °C during the RT step, so it also suits one-step RT-PCR.
| Key differences | Catalog no. by version | |||||||
|---|---|---|---|---|---|---|---|---|
| Product | Heat-labile | Digestion step | Inactivation | Recommended for | Standard | Glycerol-free | Low-residual DNA | Glycerol-free + low-residual DNA |
| UNG/UDGE01 | ✕No | 50 °C, 2 min | 95 °C, 5 min | PCR | E01 | FE01 | E01-NA | FE01-NA |
| Heat-labile UNG-V2E04-2 | Yes | 20–37 °C, 5–10 min | 50 °C, 10 min (during RT) | PCR and RT-PCR | E04-2 | FE04-2 | E04-2-NA | FE04-2-NA |
Choose Sensi Direct MD2091 to amplify straight from blood, plasma, serum, swabs or saliva, FastAmpli-V4 M2181-4 for fast cycling with 4-plex detection shown, and Superstart M2071 for standard cycling of extracted DNA.
| Key differences | Catalog no. by version | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Product | Fast cycling | Direct (extraction-free) | Inhibitor tolerant | Multiplex | Demonstrated sensitivity | Standard | Low-residual DNA | Lyo-ready | Lyo-ready + low-residual DNA |
| Superstart All-in-One qPCR MixM2071 | ✕No | ✕No | ✕No | Yes | –Not applicable or no data | M2071 | M2071-NA | FM5071 | FM5071-NA |
| FastAmpli All-in-One qPCR Mix-V4M2181-4 | Yes | ✕No | Yes | Yes4-plex shown | 3 copies/rxn (ASFV plasmid), 100% detected | M2181-4 | M2181-4-NA | FM2181-4 | FM2181-4-NA |
| Sensi Direct All-in-One qPCR MixMD2091 | Yes | Whole blood, plasma, serum, NP swab, saliva | Yes | Yes | 3 copies/rxn (ASFV plasmid), 100% detected | MD2091 | –Not applicable or no data | FMD5091 | –Not applicable or no data |
Fast Direct-V4 MD2084-4 runs straight from swabs, saliva and blood, with MgCl₂ already in the mix. FastAmpli M5144 and Neoscript Multi M8254 are for extracted RNA and supply MgCl₂ separately so you can adjust it; M5144 is also inhibitor tolerant.
| Key differences | Catalog no. by version | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Product | Fast cycling | Direct (extraction-free) | Inhibitor tolerant | Multiplex | Probe melt-curve analysis | Kit format (liquid version) | Standard | Low-residual DNA | Lyo-ready | Lyo-ready + low-residual DNA |
| Neoscript Multi RT-qPCR MixM8254 | Yes | ✕No | ✕No | Yes | Yes | 3 tubes; MgCl₂ supplied separately | M8254 | M8254-NA | FM8254 | FM8254-NA |
| FastAmpli RT-qPCR MixM5144 | Yes20–40 min | ✕No | Yes | Yes3-plex shown | Yes | 3 tubes; MgCl₂ supplied separately | M5144 | M5144-NA | FM5144 | FM5144-NA |
| Fast Direct RT-qPCR Mix-V4MD2084-4 | Yes≥1 kb/10 s | Throat swab, saliva, whole blood, plasma, serum | Yes | Yes3-plex shown | Yes | 2 tubes; MgCl₂ included in the mix | MD2084-4 | MD2084-4-NA | FMD5084-4 | –Not applicable or no data |
Pick the readout first: fluorescent dye, fluorescent probe, or a red-to-yellow color change read by eye. V3 mixes contain Bst HS-V3 (E105); the V3 dye mix also contains UNG for carry-over control. Every mix is available lyo-ready.
| Key differences | Catalog no. by version | |||||
|---|---|---|---|---|---|---|
| Product | Readout | Bst enzyme inside | UNG anti-contamination | Lyophilization data | Standard | Lyo-ready |
| LAMP Dye Mix-V2HW205-R02 | Fluorescent dye | Bst HS-V2 (E103) | ✕No | Beads = liquid performance | HW205-R02 | FHW1007-R02 |
| LAMP Dye Mix-V3HW205-R02-064803 | Fluorescent dye | Bst HS-V3 (E105) | YesTS-UNG Ⅱ | Reference protocol | HW205-R02-064803 | FHW1007-R02-064803 |
| LAMP Probe Mix-V2HW205-P01 | Fluorescent probe | Bst HS-V2 (E103) | ✕No | Beads & in-situ; 1 yr at RT | HW205-P01 | FHW1007-P01 |
| Lyo-ready LAMP Probe Mix-V3FHW1007-P01-064805 | Fluorescent probe | Bst HS-V3 (E105) | ✕No | Reference protocol | –Not applicable or no data | FHW1007-P01-064805 |
| Colorimetric LAMP Mix-V2HW205-M01 | Colorimetric (red→yellow, visual) | Bst HS-V2 (E103) | ✕No | Reference protocol | HW205-M01 | FHW1007-M01 |
The same readout choice as LAMP. The V3 dye mix FHW1008-R02-064801 pairs Bst 3.0 HS with a hot-start reverse transcriptase and is supplied lyo-ready only; the V2 mixes come in liquid and lyo-ready versions.
| Key differences | Catalog no. by version | |||||
|---|---|---|---|---|---|---|
| Product | Readout | Bst enzyme inside | Reverse transcriptase | Lyophilization data | Standard | Lyo-ready |
| RT-LAMP Dye Mix-V2HW206-R02 | Fluorescent dye | Bst HS-V2 (E103) | Neoscript RTase | Reference protocol (in-situ, 25 µL) | HW206-R02 | FHW1008-R02 |
| Lyo-ready RT-LAMP Dye Mix-V3FHW1008-R02-064801 | Fluorescent dye | Bst3.0 HS | Neoscript RTase HS (hot start) | Reference protocol (in-situ, 25 µL) | –Not applicable or no data | FHW1008-R02-064801 |
| RT-LAMP Probe Mix-V2HW206-P01 | Fluorescent probe | Bst HS-V2 (E103) | Neoscript RTase | Reference protocol (in-situ, 25 µL) | HW206-P01 | FHW1008-P01 |
| Colorimetric RT-LAMP Mix-V2HW206-M01 | Colorimetric (red→yellow, visual) | Bst HS-V2 (E103) | Neoscript RTase | Reference protocol (in-situ, 25 µL) | HW206-M01 | FHW1008-M01 |
Protectant-9 AS412-9 is the 4× protectant used in BIORI lyo-ready qPCR, RT-qPCR, LAMP and RT-LAMP mixes; the lyophilized FM2181-4 mix made with it kept its performance for 18 months at room temperature. Protectant-17 and -156-5 are 2× formulations for mostly DNA or mostly RNA systems; products freeze-dried with them tolerate 20–25% RH for 4 h before vacuum packing.
| Product | Concentration | Recommended system | Used in BIORI Lyo-ready mixes | Moisture tolerance | Lyophilized stability data |
|---|---|---|---|---|---|
| Lyophilization Protectant-9AS412-9 | 4× (6.25 µL / 25 µL rxn) | DNA and RNA systems | qPCR, RT-qPCR, LAMP, RT-LAMP | ✕No | RT 18 mo, 55 °C 30 d (lyophilized FM2181-4) |
| Lyophilization Protectant-17AS212-17 | 2× (12.5 µL / 25 µL rxn) | Mostly DNA systems | ✕No | Freeze-dried product: 20–25% RH for 4 h before vacuum packing | –Not applicable or no data |
| Lyophilization Protectant-156-5AS212-156-5 | 2× (12.5 µL / 25 µL rxn) | Mostly RNA systems | ✕No | Freeze-dried product: 20–25% RH for 4 h before vacuum packing | –Not applicable or no data |
Features are based on each product’s instruction manual, product page and data from our technical team. Prices, pack sizes and full specifications are on the product pages. Not every product is listed here; see the full range in Molecular Diagnostic Raw Materials.
Selection guides for our other product lines.
Send your chemistry, sample type and format (liquid or lyophilized). We reply within one business day with a recommendation and sample options.
Not ready for a quote? Discuss feasibility, formats, host systems or validation with the specialist behind this page — no sales script.
Topic detected: your enquiryReply within one business day from an application scientist. For pricing, COA or samples, use Request a quote instead.
Four fields. We route it to the right person and reply the way you prefer.
🔒 Used only to reply to this request. No newsletters. Privacy Policy
Need pricing, a COA or samples? Request a quote →
* Your data is securely protected for essential communications only. We won’t share your details or send spam emails, Privacy Policy *
