AcuGenix™ FastAmpli qPCR Master Mix with UNG-V4 is a dedicated reagent for qualitative and quantitative real-time PCR using probe-based chemistry. It contains a genetically engineered DNA polymerase optimized for rapid amplification, allowing the entire PCR reaction to be completed within 30 min.
The buffer system has been optimized for multiplex amplification. This reagent combines an inhibitor-tolerant amplification enzyme, UNG, and an optimized buffer system containing dUTP, enabling robust amplification of target genes in inhibitor-containing samples while effectively reducing false-positive results caused by carryover PCR products and aerosol contamination.
It is compatible with most real-time PCR instruments from Applied Biosystems, Eppendorf, Bio-Rad, and Roche.
AcuGenix™ FastAmpli qPCR Master Mix with UNG-V4, M2181-4
Features
- Dedicated for qualitative and quantitative real-time PCR using probe-based chemistry
- Contains a genetically engineered DNA polymerase optimized for rapid amplification
- Allows the entire PCR reaction to be completed within 30 min
- Buffer system optimized for multiplex amplification
- Includes inhibitor-tolerant amplification enzyme, UNG, and dUTP-containing optimized buffer system
- Effectively reduces false-positive results caused by carryover PCR products and aerosol contamination
- Compatible with most real-time PCR instruments from Applied Biosystems, Eppendorf, Bio-Rad, and Roche
Application
- Qualitative real-time PCR
- Quantitative real-time PCR
- Probe-based qPCR detection
- Multiplex real-time PCR amplification
- Highly sensitive real-time PCR detection in inhibitor-containing samples
Components
| Component | Description |
|---|---|
| AcuGenix™ FastAmpli qPCR Master Mix with UNG-V4 | This reagent already contains hot-start DNA polymerase, UNG, PCR Buffer, MgCl2, dNTPs, and stabilizers. |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20 ± 5 ℃. |
| Handling | Vortex before use. Avoid freeze-thaw cycles. |
