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PCR and qPCR mixes, reverse transcription, nucleic acid extraction and protein research reagents: the features that set each product apart, one table per family.
Fast Taq-V2 BR1A102 extends at ≥1 kb/10 s and tolerates crude templates; Taq BR1A101 runs at standard speed. Both work in multiplex PCR and probe qPCR. HandyAmp BR1A401 is a 2× fast master mix with loading dye, so products go straight onto a gel.
| Product | Format | Hot start | Fast extension | Multiplex | Crude templates / inhibitor tolerance | Usable for probe qPCR |
|---|---|---|---|---|---|---|
| Taq DNA PolymeraseBR1A101 | Enzyme only, 5 U/µL | Antibody-mediated | ✕No | Yes | ✕No | Yes |
| Fast Taq DNA Polymerase-V2BR1A102 | Enzyme only, 5 U/µL | Antibody-mediated | ≥1 kb/10 s | Yes | Yes | Yes |
| HandyAmp Fast PCR Master Mix, Dye plusBR1A401 | 2× master mix + loading dye | Engineered hot-start enzyme | 1 s/kb (≤2 kb) | 5-plex | Yes | ✕No |
First choose dye or probe detection; the probe mixes support multiplexing. The FastAmpli mixes run a ~30 min program and tolerate inhibitors; take a "with UNG" version BR1E103, BR1E202 for dUTP/UNG carry-over control. Robustart BR1E101 runs the standard program.
| Product | Detection chemistry | Fast cycling (~30 min run) | UNG/dUTP anti-contamination | Multiplex | Inhibitor tolerance |
|---|---|---|---|---|---|
| Robustart qPCR Mix, dyeBR1E101 | Dye (SYBR-type) | ✕No | ✕No | N/A (dye-based) | ✕No |
| FastAmpli qPCR Mix, dyeBR1E102 | Dye (SYBR-type) | Yes | ✕No | N/A (dye-based) | Yes |
| FastAmpli qPCR Mix with UNG, dyeBR1E103 | Dye (SYBR-type) | Yes | Yes | N/A (dye-based) | Yes |
| FastAmpli Probe qPCR MixBR1E203 | Probe | Yes | ✕No | Yes | Yes |
| FastAmpli Probe qPCR Mix with UNGBR1E202 | Probe | Yes | Yes | Yes | Yes |
Choose dye or probe detection, then the option you need: the Neoscript Fast dye mix BR1F103 has the 5-min RT step; the "with UNG" mixes BR1F102, BR1F202 add TS-UNG for dUTP/UNG carry-over control.
| Product | Detection chemistry | Fast protocol (5 min RT) | UNG/dUTP anti-contamination |
|---|---|---|---|
| RT qPCR Mix, dyeBR1F101 | Dye (SYBR-type) | ✕No | ✕No |
| RT qPCR Mix with UNG, dyeBR1F102 | Dye (SYBR-type) | ✕No | YesTS-UNG |
| Neoscript Fast RT qPCR Mix, dyeBR1F103 | Dye (SYBR-type) | Yes | ✕No |
| RT Probe qPCR MixBR1F201 | Probe | ✕No | ✕No |
| RT Probe qPCR Mix with UNGBR1F202 | Probe | ✕No | YesTS-UNG |
Reverse Transcriptase BR1D101 is the enzyme alone, for building your own RT-PCR, cDNA library or RACE workflow. Both kits remove gDNA and include a No-RT control: the 1st cDNA Synthesis Kit BR1D201 keeps oligo(dT) and random primers in separate tubes, while the cDNA Synthesis Master Mix BR1D301 is premixed for two-step RT-qPCR.
| Product | Format | RT primers | gDNA removal + No-RT control | RT conditions | Best for |
|---|---|---|---|---|---|
| Reverse Transcriptase, 200 U/µLBR1D101 | Enzyme only, 200 U/µL (buffer, dNTPs, primers, RNase inhibitor not included) | Not included | ✕No | 50 °C, 30–60 min (42–55 °C); random primers: 25 °C, 10 min first | RT-PCR/qPCR, one-step RT-qPCR assay building, cDNA library, 3′/5′ RACE |
| Neoscript 1st cDNA Synthesis Kit + gDNA WiperBR1D201 | Kit: 4× RT core mix + separate primer tubes | Oligo(dT)18 VN + Random 6-mers (separate tubes) | Yes | PCR: 50 °C 5 min; qPCR: 37 °C 15 min; then 85 °C 5 s | Conventional PCR & two-step RT-qPCR |
| Neoscript cDNA Synthesis Master Mix for qPCR + gDNA WiperBR1D301 | 5× all-in-one master mix | Oligo(dT)18 + Random 6-mers (premixed) | Yes | 50 °C 15 min, 85 °C 5 s | Two-step RT-qPCR (dye & probe) |
Choose by what you isolate: plasmid DNA (miniprep BR2D101, or the low-endotoxin maxiprep BR2D102 for transfection), DNA fragments from gels or PCR BR2E301, total RNA with Brizol BR2B101, or DNA and RNA from biological-product intermediates on magnetic beads, validated on automated instruments BP-QN11-100.
| Product | Isolates | Starting material | Method | Automation-compatible | Endotoxin-free (transfection grade) | Scale / capacity |
|---|---|---|---|---|---|---|
| Plasmid Miniprep KitBR2D101 | Plasmid DNA | 1–5 mL overnight E. coli culture | Silica spin column | ✕No | ✕No | Up to 30 µg per column |
| EndoFree Plasmid Maxi KitBR2D102 | Plasmid DNA | 100–200 mL overnight bacterial culture | Silica spin column | ✕No | YesLow endotoxin, transfection grade | 500–1,500 µg from 100 mL high-copy culture |
| Universal DNA Purification Kit, gel & PCR cleanupBR2E301 | DNA fragments (gel, PCR, digests) | TAE/TBE agarose gel, PCR or digestion reactions | Spin column | ✕No | –Not applicable or no data | 100 bp–10 kb, up to 80% recovery |
| Brizol Reagent, total RNABR2B101 | Total RNA | Tissues, cells, blood, plants, microbes, viruses | Phenol reagent (chloroform phase separation) | ✕No | –Not applicable or no data | 1 mL per 15–50 mg tissue |
| Nucleic Acid Extraction Kit II, magnetic beadsBP-QN11-100 | DNA & RNA | Biological-product intermediate samples | Magnetic beads | YesBiori extraction system & validated instruments | –Not applicable or no data | Sample ≤400 µL |
Strong RIPA BR4C101 is rated excellent for membrane and nuclear proteins; Weak RIPA BR4C103 and the Cell Total Protein Extraction Kit BR4C131 are Co-IP compatible. The kit also includes protease inhibitor and loading buffer, and covers animal cells, bacteria and fungi (no tissue protocol).
| Product | Lysis strength | Key detergents | Membrane proteins | Nuclear proteins | Co-IP compatible | Sample types | Inhibitor + loading buffer included |
|---|---|---|---|---|---|---|---|
| RIPA Lysis Buffer, StrongBR4C101 | Strong | 1% Triton X-100, 0.75% sodium deoxycholate, 0.1% SDS | Excellent | Excellent | ✕No | Animal cells & tissues, bacteria, yeast, plant cells | ✕No |
| RIPA Lysis Buffer, MediumBR4C102 | Medium | 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS | Good | Good | ✕No | Animal cells & tissues, bacteria, yeast, plant cells | ✕No |
| RIPA Lysis Buffer, WeakBR4C103 | Weak | 1% NP-40, 0.05% sodium deoxycholate | Average | Good | Yes | Animal cells & tissues, bacteria, yeast, plant cells | ✕No |
| Cell Total Protein Extraction KitBR4C131 | Total protein | 1% NP-40, 0.05% sodium deoxycholate | Yes | Yes | Yes | Animal cells, bacteria, fungi (no tissue protocol) | Yes |
Bradford BR4C202 is the quick one: 3–5 min at room temperature, 10 µL sample, ready-to-use standards, and it tolerates common detergents below 2%. BCA BR4C201 reaches lower concentrations, from 20 µg/mL, with a 30-min incubation at 37 °C, but does not tolerate detergents.
| Product | Assay principle (read-out) | Working range | Incubation | Sample per well | Detergent limit | Ready-to-use standards |
|---|---|---|---|---|---|---|
| BCA Protein Assay KitBR4C201 | BCA–Cu⁺ chelation (A562) | 20–300 µg/mL (standard curve) | 37 °C, 30 min | 50 µL | Not tolerated | ✕No1 mg/mL BSA stock, user dilutes |
| Bradford Protein Assay KitBR4C202 | Coomassie G-250 dye binding (A595) | 100–500 µg/mL | RT, 3–5 min | 10 µL | <2% SDS, Triton X-100, Tween-20, NP-40, Brij35 | Yes7 levels, 0–500 µg/mL |
Pick the gel percentage by protein size, from 6% for 50–250 kDa to 15% for 10–70 kDa; the 10% kit BR4C333 is the general-purpose choice.
| Product | Separating gel | Resolving range | Best for |
|---|---|---|---|
| One-Step PAGE Gel Kit 6%BR4C331 | 6% | 50–250 kDa | High-molecular-weight proteins |
| One-Step PAGE Gel Kit 7.5%BR4C332 | 7.5% | 30–200 kDa | Medium- to high-molecular-weight proteins |
| One-Step PAGE Gel Kit 10%BR4C333 | 10% | 20–150 kDa | General-purpose protein separation |
| One-Step PAGE Gel Kit 12.5%BR4C334 | 12.5% | 10–100 kDa | Medium- to low-molecular-weight proteins |
| One-Step PAGE Gel Kit 15%BR4C335 | 15% | 10–70 kDa | Low-molecular-weight proteins |
Use 0.22 µm membranes for proteins under 20 kDa and 0.45 µm for larger ones. NC needs only water and gives low background for fluorescent detection; PVDF needs methanol or ethanol activation, has a stated binding capacity of >200 µg/cm² (NC: >115 µg/cm²) and can be stripped and reprobed.
| Product | Material | Pore size | Recommended protein size | Pre-wetting | Binding capacity | Fluorescent detection | Stripping & reprobing |
|---|---|---|---|---|---|---|---|
| NC Membrane, 0.22 µmBR4D414 | Nitrocellulose (NC) | 0.22 µm | < 20 kDa | Water only | >115 µg/cm² | YesLow background | ✕NoNot recommended |
| NC Membrane, 0.45 µmBR4D413 | Nitrocellulose (NC) | 0.45 µm | ≥ 20 kDa | Water only | >115 µg/cm² | YesLow background | ✕NoNot recommended |
| PVDF Membrane, 0.22 µmBR4D412 | PVDF | 0.22 µm | < 20 kDa | Methanol/ethanol activation | >200 µg/cm² | ✕NoHigh background | Yes |
| PVDF Membrane, 0.45 µmBR4D411 | PVDF | 0.45 µm | ≥ 20 kDa | Methanol/ethanol activation | >200 µg/cm² | ✕NoHigh background | Yes |
Features are based on each product’s instruction manual, product page and data from our technical team. Prices, pack sizes and full specifications are on the product pages. Not every product is listed here; see the full range in Life Science.
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