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AcuGenix™ Hot Start Taq DNA Polymerase is a chemically modified hot-start enzyme designed to prevent non-specific amplification and primer-dimer formation. Ideal for high-specificity PCR and qPCR applications.
AcuGenix™ Hot Start Taq DNA Polymerase, E03
USD
$0.00
Cat. No.: E03
Features
- Chemically modified hot-start Taq polymerase requiring activation at 95°C for 5–10 minutes.
- Completely blocks polymerase activity at room temperature to suppress non-specific amplification.
- Time-release activation mechanism gradually restores enzyme activity during PCR cycling.
- Improves amplification specificity and sensitivity, especially for low-copy templates.
- Delivers strong fluorescence signals and reliable performance in qPCR assays.
Application
- Conventional PCR amplification
- Quantitative PCR (qPCR) assays
- Multiplex PCR detection
- Genotyping and SNP analysis
- Low-copy DNA template amplification
Components
| No. | Component | Notes |
|---|---|---|
| 1 | 5 U/µL AcuGenix™ Hot Start Taq DNA Polymerase | — |
| 2 | 10× HS Buffer (Mg²⁺-free) | Optional |
| 3 | 25 mM MgCl₂ | Optional |
Note: The 10× HS Buffer (Mg²⁺-free) does not contain dNTPs or Mg²⁺. Please add dNTPs and MgCl₂ when preparing the reaction system.
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Shipping | Ship on dry ice. |
| Storage | Store at −20 ± 5°C. |
| Handling | Mix thoroughly before use and avoid repeated freeze–thaw cycles. |
Data
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