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Product Catalog
CHO Residual DNA Detection Kit (qPCR), BP-QN06, is designed for quantitative detection of residual CHO host-cell DNA in intermediates, semi-finished products, and finished biological or pharmaceutical products. The kit uses fluorescent probe qPCR with an internal standard to support extraction and amplification monitoring during bioprocess quality-control workflows.
CHO Residual DNA Detection Kit (qPCR), BP-QN06
USD
$0.00
BP-QN06-100
Features
- qPCR probe-based assay for conserved CHO cell sequences.
- Includes an exogenous internal standard to monitor extraction and amplification performance.
- UDG enzyme is included to help prevent amplification-product contamination.
- Six CHO calibrators are supplied from 3 fg/uL to 300 pg/uL.
- COA reports conforming precision, accuracy, amplification efficiency, linear correlation, and internal-standard Ct results.
Application
- Quantification of residual CHO host-cell DNA in biopharmaceutical process samples.
- Testing of intermediates, semi-finished products, and finished products.
- qPCR workflows using supported real-time PCR systems listed in the manual.
- Extraction and recovery-control workflows when paired with a suitable nucleic acid extraction or purification kit.
Components
| Component | Specification |
|---|---|
| CHO qPCR MIX | 1.0 mL x 2 tubes |
| CHO Internal Standard | 1.0 mL x 1 tube |
| CHO Negative Control | 1.0 mL x 2 tubes |
| CHO Calibrator ST1 | 0.5 mL x 1 tube; 3 fg/uL |
| CHO Calibrator ST2 | 0.5 mL x 1 tube; 30 fg/uL |
| CHO Calibrator ST3 | 0.5 mL x 1 tube; 300 fg/uL |
| CHO Calibrator ST4 | 0.5 mL x 1 tube; 3 pg/uL |
| CHO Calibrator ST5 | 0.5 mL x 1 tube; 30 pg/uL |
| CHO Calibrator ST6 | 0.5 mL x 1 tube; 300 pg/uL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | <= -20 C, protected from light. Shelf life: 24 months. Avoid more than 10 freeze-thaw cycles. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Protect from light and avoid unnecessary freeze-thaw cycles where applicable. |
Data
COA lot 20251111 reports conforming negative concordance, precision, accuracy, amplification efficiency of 95-105%, linear correlation |r| >= 0.99, internal-standard Ct < 35, and overall compliance with the enterprise standard.
