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Product Catalog
DNase I GMP-grade (Deoxyribonuclease I) is an endonuclease that efficiently digests single-stranded and double-stranded DNA. DNase I requires Ca²⁺ for activity and is activated by Mg²⁺ or Mn²⁺.
In the presence of Mg²⁺, DNase I cleaves DNA at random sites; in the presence of Mn²⁺, the enzyme cleaves both strands of double-stranded DNA at approximately the same position, generating blunt-ended fragments or fragments with 1–2 nucleotide overhangs.
DNase I GMP-grade exhibits optimal endonuclease activity at pH 7–8 and is suitable for removing single-stranded or double-stranded DNA from RNA preparations and other downstream workflows.
Features
- Efficiently digests single-stranded and double-stranded DNA
- Requires Ca²⁺ for activity and is activated by Mg²⁺ or Mn²⁺
- In the presence of Mg²⁺, cleaves DNA at random sites
- In the presence of Mn²⁺, generates blunt-ended fragments or fragments with 1–2 nucleotide overhangs
- Optimal endonuclease activity at pH 7–8
- Suitable for removing residual DNA from RNA preparations
- Supplied with 10× DNase I Reaction Buffer for convenient use
Application
- Preparation of DNA-free RNA samples for downstream RNA analysis
- Digestion of linearized plasmid template following in vitro transcription
- Removal of residual genomic DNA prior to RT-PCR
- DNase I footprinting analysis of DNA–protein interactions
Components
| Component | GMP-BP-E04-200 | GMP-BP-E04-2K | GMP-BP-E04-20K |
|---|---|---|---|
| DNase I GMP-grade (2 U/µL) | 200 U / 0.1 mL | 2,000 U / 1 mL | 20,000 U / 10 mL |
| 10× DNase I Reaction Buffer | 0.2 mL | 2 mL | 20 mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at −20 ± 5 °C. |
| Handling | Mix gently before use. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | 10 mM Tris, 2 mM CaCl₂, 50% (v/v) glycerol, pH 7.6 |
| Inactivation | Add EDTA to a final concentration of 5 mM and incubate at 65 °C for 10 min to inactivate the enzyme. |
Data
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