Additional information
| Size | 20KU, 200U, 2000U |
|---|
DNase I GMP-grade, GMP-BP-E04
Price range: $20.00 through $960.00
DNase I GMP-grade (Deoxyribonuclease I) is an endonuclease that efficiently digests single-stranded and double-stranded DNA. DNase I requires Ca²⁺ for activity and is activated by Mg²⁺ or Mn²⁺.
In the presence of Mg²⁺, DNase I cleaves DNA at random sites; in the presence of Mn²⁺, the enzyme cleaves both strands of double-stranded DNA at approximately the same position, generating blunt-ended fragments or fragments with 1–2 nucleotide overhangs.
DNase I GMP-grade exhibits optimal endonuclease activity at pH 7–8 and is suitable for removing single-stranded or double-stranded DNA from RNA preparations and other downstream workflows.
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