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Product Catalog
Vaccinia Capping Enzyme GMP-grade provides the enzymatic activities required for RNA 5′ capping, including RNA triphosphatase, guanylyltransferase, and guanine-N7-methyltransferase activities. In the presence of GTP and SAM, it supports enzymatic formation of Cap0-capped RNA according to the protocol.
Vaccinia Capping Enzyme GMP-grade, GMP-BP-E05
USD
$0.00
GMP-BP-E05
Features
- GMP-grade enzyme for in vitro transcription and mRNA manufacturing workflows.
- Instruction manual provides activity, storage, and quality-control information for the enzyme.
- Designed for use in RNA synthesis, template preparation, or post-transcriptional processing workflows as applicable.
Application
- In vitro transcription and mRNA process development workflows.
- Research or manufacturing workflows requiring the specified GMP-grade enzyme.
Components
| Components | Volume |
|---|---|
| 10× Capping Buffer | 2uL |
| GTP Solution (10mM) | 1uL |
| SAM (2mM) | 1uL |
| Vaccinia Capping Enzyme GMP-grade (10 U/uL) | 1uL |
| RNase Inhibitor GMP-grade (40 U/uL) | 0.5uL |
| Denatured RNA | 50pmol |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
Data
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