Vaccinia Capping Enzyme GMP-grade, GMP-BP-E05

Mature mRNA in eukaryotes carries a unique 5′ cap structure (m⁷GpppN), known as the methylguanosine cap, which is essential for translation initiation, protection from RNase degradation, and enhancement of mRNA stability during splicing and nuclear export.

Vaccinia Capping Enzyme GMP-grade (VCE), developed in-house by Biori Biotech, integrates the three enzymatic activities required for capping: RNA triphosphatase, guanylyltransferase, and guanine-N7-methyltransferase.

In the presence of S-adenosylmethionine (SAM) as a methyl donor and guanosine triphosphate (GTP), VCE directly and efficiently adds an m⁷G cap to the 5′ end of mRNA in the correct orientation, producing Cap0-capped RNA with up to 100% capping efficiency in a single reaction.