Mature mRNA in eukaryotes carries a unique 5′ cap structure (m⁷GpppN), known as the methylguanosine cap, which is essential for translation initiation, protection from RNase degradation, and enhancement of mRNA stability during splicing and nuclear export.
Vaccinia Capping Enzyme GMP-grade (VCE), developed in-house by Biori Biotech, integrates the three enzymatic activities required for capping: RNA triphosphatase, guanylyltransferase, and guanine-N7-methyltransferase.
In the presence of S-adenosylmethionine (SAM) as a methyl donor and guanosine triphosphate (GTP), VCE directly and efficiently adds an m⁷G cap to the 5′ end of mRNA in the correct orientation, producing Cap0-capped RNA with up to 100% capping efficiency in a single reaction.
Vaccinia Capping Enzyme GMP-grade, GMP-BP-E05
Features
- Integrates RNA triphosphatase, guanylyltransferase, and guanine-N7-methyltransferase activities in one enzyme
- Directly adds an m⁷G cap to the 5′ end of mRNA in the correct orientation
- Produces Cap0-capped RNA with up to 100% capping efficiency in a single reaction
- Compatible with GTP and SAM-mediated enzymatic capping workflows
- Developed in-house by Biori Biotech
- Cap1 RNA can be obtained by combining with 2′-O-Methyltransferase GMP-grade (GMP-BP-E06)
Application
- Cap 0 capping of mRNA for in vitro or in vivo translation
- Generation of Cap1 RNA when used in combination with 2′-O-Methyltransferase GMP-grade (GMP-BP-E06)
Components
| Component | GMP-BP-E05-1K | GMP-BP-E05-10K | GMP-BP-E05-100K |
|---|---|---|---|
| Vaccinia Capping Enzyme GMP-grade (10 U/µL) | 1,000 U / 0.1 mL | 10,000 U / 1 mL | 100,000 U / 10 mL |
| 10× Capping Buffer | 0.2 mL | 2 mL | 20 mL |
| GTP Solution (10 mM) | 0.1 mL | 1 mL | 10 mL |
| SAM (32 mM) | 20 µL | 0.2 mL | 2 mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at −20 ± 5 °C. |
| Handling | Mix gently before use. Avoid repeated freeze-thaw cycles. |
| Capping Buffer | 500 mM Tris-HCl (pH 8.0), 50 mM KCl, 10 mM MgCl₂, 10 mM DTT |
| Storage Buffer | 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% (v/v) Triton X-100, 50% (v/v) glycerol |
Data
Coming Soon…
