Neoscript Multi One-Step RT-qPCR Master Mix with UNG is a dedicated reagent for one-step qualitative and quantitative RT-qPCR using the probe method and is suitable for multiplex amplification. The entire reaction is performed sequentially in a single tube, avoiding tube opening and effectively reducing contamination.

The product contains Neoscript RTase, a thermostable reverse transcriptase with reduced RNase H activity, and a genetically engineered hot-start Taq DNA polymerase, providing higher reverse transcription and PCR amplification efficiency and making it suitable for highly sensitive amplification of low-concentration RNA templates.

The reagent uses an optimized qPCR buffer system together with a UNG/dUTP anti-contamination system, enabling good standard curves over a broad dynamic range for accurate quantification while effectively preventing false-positive amplification caused by residual PCR products or aerosol contamination.