Product Catalog
RNase Inhibitor GMP-grade is a recombinant RNase inhibitor produced through recombinant expression and multi-step purification. It inhibits RNase A, RNase B, and RNase C activity through high-affinity non-covalent 1:1 binding, while having no effect on RNase H or S1 Nuclease activity.
This product does not inhibit phage RNA polymerases (SP6, T7, or T3), Taq DNA polymerase, AMV reverse transcriptase, or M-MLV reverse transcriptase. RNase Inhibitor GMP-grade exhibits enhanced oxidative stability and maintains activity under low DTT conditions.
It is suitable for excluding RNase contamination in mRNA in vitro transcription, storage, purification, reverse transcription, qPCR, and other RNA-related applications requiring intact RNA.
Features
- Recombinant RNase inhibitor produced through recombinant expression and multi-step purification
- Inhibits RNase A, RNase B, and RNase C through high-affinity non-covalent 1:1 binding
- Does not inhibit RNase H or S1 Nuclease activity
- Compatible with phage RNA polymerases (SP6, T7, and T3), Taq DNA polymerase, AMV reverse transcriptase, and M-MLV reverse transcriptase
- Enhanced oxidative stability
- Maintains activity under low DTT conditions
- Suitable for excluding RNase contamination in mRNA in vitro transcription and related applications
Application
- In vitro transcription
- mRNA storage and transport
- mRNA isolation and purification
- Reverse transcription and qPCR
- Applications requiring intact RNA
Components
| No. | Component | Catalog No. | Quantity | Volume |
|---|---|---|---|---|
| 1 | RNase Inhibitor GMP-grade (40 U/µL) | GMP-BP-E02-4K | 4 KU | 0.1 mL |
| 2 | RNase Inhibitor GMP-grade (40 U/µL) | GMP-BP-E02-40K | 40 KU | 1 mL |
| 3 | RNase Inhibitor GMP-grade (40 U/µL) | GMP-BP-E02-400K | 400 KU | 10 mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20 ± 5 °C. |
| Handling | Vortex before use. Avoid repeated freeze-thaw cycles. |
Data
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