Utility Enzymes
Fast-cut restriction endonucleases that digest in 5–15 min in the universal 10× Cut-Buffer, plus heat-labile UNG and FEN1.
Which product for which use
The product we would pick first for each use, with alternatives from the same group.
| Use case | Recommended product | Why this one | Alternatives |
|---|---|---|---|
| Restriction Endonucleases | AatII BR1G133 Data | AatII fast-cut restriction endonuclease (recognition site GACGT↓C) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer. | BR1G102, BR1G153, BR1G144, BR1G136, BR1G131, BR1G161, BR1G126, BR1G158, BR1G137, BR1G127, BR1G159, BR1G110, BR1G156, BR1G106, BR1G109, BR1G107, BR1G108, BR1G130, BR1G129, BR1G122, BR1G157, BR1G134, BR1G111, BR1G149, BR1G148, BR1G143, BR1G146, BR1G141, BR1G113, BR1G138, BR1G103, BR1G135, BR1G105, BR1G154, BR1G162, BR1G160, BR1G121, BR1G117, BR1G140, BR1G112, BR1G118, BR1G119, BR1G164, BR1G139, BR1G132, BR1G163, BR1G147, BR1G142, BR1G145, BR1G125, BR1G115, BR1G124, BR1G152, BR1G128, BR1G151, BR1G150, BR1G155, BR1G123, BR1G116 |
| Modifying Enzymes | Heat-labile Uracil-N-Glycosylase (UNG) BR1A808 | Heat-labile UNG for dUTP-based PCR carryover control. | — |
| Nucleases | FEN1 BR1G101 | Flap endonuclease 1 (FEN1) that cleaves 5′ flap structures in DNA, for flap-based probe cleavage assays and enzymatic DNA processing in research workflows. | — |
Products
AatII
AatII fast-cut restriction endonuclease (recognition site GACGT↓C) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
AciI
AciI fast-cut restriction endonuclease (recognition site C↓CGC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
AflII
AflII fast-cut restriction endonuclease (recognition site C↓TTAAG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
AluI
AluI fast-cut restriction endonuclease (recognition site AG↓CT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
ApaLI
ApaLI fast-cut restriction endonuclease (recognition site G↓TGCAC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
AscI
AscI fast-cut restriction endonuclease (recognition site GG↓CGCGCC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
AvrII
AvrII fast-cut restriction endonuclease (recognition site C↓CTAGG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BamHI
BamHI fast-cut restriction endonuclease (recognition site G↓GATCC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BclI
BclI fast-cut restriction endonuclease (recognition site T↓GATCA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BglI
BglI fast-cut restriction endonuclease (recognition site GCCNNNN↓NGGC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BglII
BglII fast-cut restriction endonuclease (recognition site A↓GATCT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BsaHI
BsaHI fast-cut restriction endonuclease (recognition site GR↓CGYC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BsaI
BsaI fast-cut restriction endonuclease (recognition site GGTCTC(N) ↓) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BsiWI
BsiWI fast-cut restriction endonuclease (recognition site C↓GTACG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BspEI
BspEI fast-cut restriction endonuclease (recognition site T↓CCGGA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BspQI
BspQI fast-cut restriction endonuclease (recognition site GCTCTTC(N)↓) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BsrFI
BsrFI fast-cut restriction endonuclease (recognition site R↓CCGGY) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BssHII
BssHII fast-cut restriction endonuclease (recognition site G↓CGCGC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BstEII
BstEII fast-cut restriction endonuclease (recognition site G↓GTNACC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
ClaI
ClaI fast-cut restriction endonuclease (recognition site AT↓CGAT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
DpnI
DpnI fast-cut restriction endonuclease (recognition site GAᵐ⁶↓TC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
DpnII
DpnII fast-cut restriction endonuclease (recognition site ↓GATC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
DraI
DraI fast-cut restriction endonuclease (recognition site TTT↓AAA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
EcoRI
EcoRI fast-cut restriction endonuclease (recognition site G↓AATTC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
EcoRV
EcoRV fast-cut restriction endonuclease (recognition site GAT↓ATC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
EcoT22I
EcoT22I fast-cut restriction endonuclease (recognition site ATGCA↓T) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
FokI
FokI fast-cut restriction endonuclease (recognition site GGATG(N)₉↓) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
FspI
FspI fast-cut restriction endonuclease (recognition site TGC↓GCA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HaeIII
HaeIII fast-cut restriction endonuclease (recognition site GG↓CC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HindIII
HindIII fast-cut restriction endonuclease (recognition site A↓AGCTT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HinfI
HinfI fast-cut restriction endonuclease (recognition site G↓ANTC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HinP1I
HinP1I fast-cut restriction endonuclease (recognition site G↓CGC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HpaI
HpaI fast-cut restriction endonuclease (recognition site GTT↓AAC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HpaII
HpaII fast-cut restriction endonuclease (recognition site C↓CGG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
KasI
KasI fast-cut restriction endonuclease (recognition site G↓GCGCC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
KpnI
KpnI fast-cut restriction endonuclease (recognition site GGTAC↓C) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
MboI
MboI fast-cut restriction endonuclease (recognition site ↓GATC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
MluI
MluI fast-cut restriction endonuclease (recognition site A↓CGCGT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
MscI
MscI fast-cut restriction endonuclease (recognition site TGG↓CCA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
MunI
MunI fast-cut restriction endonuclease (recognition site C↓AATTG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
NaeI
NaeI fast-cut restriction endonuclease (recognition site GCC↓GGC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
NcoI
NcoI fast-cut restriction endonuclease (recognition site C↓CATGG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
NdeI
NdeI fast-cut restriction endonuclease (recognition site CA↓TATG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
NruI
NruI fast-cut restriction endonuclease (recognition site TCG↓CGA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
PmeI
PmeI fast-cut restriction endonuclease (recognition site GTTT↓AAAC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
PmlI
PmlI fast-cut restriction endonuclease (recognition site CAC↓GTG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
PspGI
PspGI fast-cut restriction endonuclease (recognition site ↓CCWGG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
PstI
PstI fast-cut restriction endonuclease (recognition site CTGCA↓G) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
PvuII
PvuII fast-cut restriction endonuclease (recognition site CAG↓CTG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
RsaI
RsaI fast-cut restriction endonuclease (recognition site GT↓AC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SacII
SacII fast-cut restriction endonuclease (recognition site CCGC↓GG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SalI
SalI fast-cut restriction endonuclease (recognition site G↓TCGAC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
ScaI
ScaI fast-cut restriction endonuclease (recognition site AGT↓ACT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SdaI
SdaI fast-cut restriction endonuclease (recognition site CCTGCA↓GG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SmaI
SmaI fast-cut restriction endonuclease (recognition site CCC↓GGG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SnaBI
SnaBI fast-cut restriction endonuclease (recognition site TAC↓GTA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SspI
SspI fast-cut restriction endonuclease (recognition site AAT↓ATT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
TaqI
TaqI fast-cut restriction endonuclease (recognition site T↓CGA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
XbaI
XbaI fast-cut restriction endonuclease (recognition site T↓CTAGA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
XhoI
XhoI fast-cut restriction endonuclease (recognition site C↓TCGAG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
FEN1
Flap endonuclease 1 (FEN1) that cleaves 5′ flap structures in DNA, for flap-based probe cleavage assays and enzymatic DNA processing in research workflows.
Related categories
Nucleic Acid ElectrophoresisPCRqPCRReverse TranscriptionRT-qPCR
Documents
Manuals, COA and SDS are linked on each product row and on the product page.
Custom and OEM
Bulk fills, custom formulations and pilot evaluation are quoted per project.
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