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Product Catalog
Robustart Fast Taq DNA Polymerase-V3 is a hot-start rapid amplification Taq enzyme optimized for freeze-dried reagent systems. It effectively suppresses non-specific amplification caused by primer misannealing or primer-dimer formation during PCR setup and amplification, delivering high specificity and improved performance for low-concentration templates. With good system adaptability, it provides stable amplification results in different PCR reaction types and is suitable for multiplex PCR amplification.
Robustart Fast Taq DNA Polymerase-V3,E40
USD
$0.00
Cat. No.: E40
Features
- Antibody-modified hot-start enzyme, activated at 95°C for 1–5 minutes.
- Fast amplification speed of no less than 1 kb/10 s.
- Effectively suppresses non-specific amplification caused by primer misannealing and primer-dimer formation.
- High specificity and improved performance for low-concentration templates.
- Strong tolerance to inhibitors from blood, swabs, and residual substances such as phenols and alcohols from sample pretreatment.
- Good system adaptability with stable amplification performance across different PCR reaction types.
- Suitable for multiplex PCR amplification.
Application
- Endpoint PCR amplification
- Quantitative PCR assays
- Rapid PCR amplification
- Direct PCR amplification
- Multiplex PCR amplification
- Nucleic acid testing applications in POCT (Point-of-Care Testing)
- Suitable for ordinary PCR and RT-PCR detection
Components
| No. | Component | Notes |
|---|---|---|
| 1 | 5 U/μL Robustart Fast Taq DNA Polymerase-V3 | — |
| 2 | 10× PCR Buffer II (Mg2+-free) | Optional |
| 3 | 25 mM MgCl2 | Optional |
Note: The 10× PCR Buffer II (Mg2+-free) does not contain dNTPs or Mg2+. Please add dNTPs and MgCl2 when preparing the reaction system.
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Shipping | Ship on dry ice. |
| Storage | Store at −20 ± 5°C. |
| Handling | Mix thoroughly before use and avoid repeated freeze–thaw cycles. |
Data
Experimental validation data will be updated soon.
