Robustart Taq DNA Polymerase is a hot-start PCR enzyme developed for high-specificity DNA amplification. The enzyme effectively suppresses non-specific reactions caused by primer misannealing or primer-dimer formation during PCR setup and amplification.
It provides improved amplification efficiency for low-concentration DNA templates and demonstrates strong system adaptability across various PCR assays. Robustart Taq DNA Polymerase is suitable for conventional PCR, quantitative PCR, and multiplex PCR applications, delivering reliable and stable amplification performance.
Robustart Taq DNA Polymerase, E16
Features
- Antibody-modified hot-start activation at 95°C for 1–5 minutes.
- High sensitivity and strong amplification specificity.
- Efficient amplification of low-copy DNA templates.
- Excellent compatibility across various PCR reaction systems.
- Reliable performance in multiplex PCR assays.
Application
- Conventional PCR amplification
- Quantitative PCR (qPCR) assays
- Multiplex PCR detection
- Genotyping and SNP analysis
- High-sensitivity pathogen detection
Components
| No. | Component | Notes |
|---|---|---|
| 1 | 5 U/µL Robustart Taq DNA Polymerase | — |
| 2 | 10× PCR Buffer II (Mg²⁺-free) | Optional |
| 3 | 25 mM MgCl₂ | Optional |
Note: The 10× PCR Buffer II (Mg²⁺-free) does not contain dNTPs or Mg²⁺. Please add dNTPs and MgCl₂ when preparing the PCR reaction system.
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Shipping | Ship on dry ice. |
| Storage | Store at −20 ± 5°C. |
| Handling | Mix thoroughly before use and avoid repeated freeze–thaw cycles. |
Data
Experimental validation data will be updated soon.
