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Product Catalog
SeamLess Cloning Master Mix is a simple, rapid, and efficient universal seamless cloning reagent developed by Biori. It enables rapid directional cloning of insert fragments into any site of virtually any vector.
Designed for high cloning efficiency, this master mix delivers high colony numbers and high positive rates in homologous recombination-based assembly workflows. It supports the assembly of up to 15 insert fragments in a single reaction, making it suitable for both routine cloning and more complex construct design.
SeamLess Cloning Master Mix is ideal for rapid cloning, high-throughput cloning, seamless cloning, and DNA site-directed mutagenesis workflows in molecular biology research.
Features
- Universal seamless cloning reagent for directional cloning into virtually any vector site.
- Supports efficient homologous recombination with high colony numbers and high positive rates.
- Enables assembly of up to 15 insert fragments in a single reaction.
- Suitable for rapid cloning, high-throughput cloning, seamless cloning, and DNA site-directed mutagenesis.
- Compatible with insert fragments prepared by restriction digestion or PCR amplification.
- Optimized for flexible single-insert and multi-insert assembly workflows.
Application
- Rapid cloning
- High-throughput cloning
- Seamless cloning
- Directional cloning of insert fragments into vectors
- Single-insert and multi-insert DNA assembly
- DNA site-directed mutagenesis
Components
| Component | BR1C101-01 | BR1C101-02 | BR1C101-03 |
|---|---|---|---|
| Pack Size | 20 T | 40 T | 20 T × 5 |
| SeamLess Cloning Master Mix | 0.1 mL | 0.2 mL | 0.2 mL × 5 |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5℃. |
| Handling | Vortex before use. Avoid repeated freeze-thaw cycles. |
| Post-Reaction Handling | After the reaction, place on ice and transform immediately. If immediate transformation is not possible, store at -20℃. |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Data
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