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Multiplex PCR for targeted NGS, library prep kit and modules, NGS enzymes, high-fidelity and long-range PCR, and extraction: the features that set each product apart, one table per family.
Choose by template: the Mul-PCR Kit BR3M106 is for DNA panels of 10–600-plex; the Mul-PCR Mix for DNA&RNA BR3M303 amplifies DNA and RNA targets in one tube; the AmpHiFi Mul-RT-PCR Kit BR3M322 is for RNA panels and adds proofreading, about 68× the fidelity of Taq.
| Product | Template / RT step | Validated plexity | Fidelity vs. Taq | Hot-start mechanism | Supplied format | Template input | GC range |
|---|---|---|---|---|---|---|---|
| Mul-PCR KitBR3M106 | DNA only | 10–600-plex | Taq-based (no proofreading) | Antibody | 2× buffer + separate enzyme | 0.1–500 ng DNA | 27–80% |
| Mul-PCR Mix for DNA&RNABR3M303 | DNA + RNA (one-tube RT-PCR) | 400+-plex | Taq-based (no proofreading) | Chemically modified | Single-tube 2× mix | 0.1–500 ng | –Not applicable or no data |
| AmpHiFi Mul-RT-PCR KitBR3M322 | RNA (one-tube RT-PCR) | 400+-plex | ~68× (proofreading) | Antibody | 2× buffer + separate enzyme | 0.1–200 ng RNA | 25–85% |
The Universal DNA Library Prep Kit BR3D201 covers fragmentation, end repair, dA-tailing, adapter ligation and library amplification; adapters and clean-up beads are not included. The modules replace single steps: fragmentation BR3N602, library amplification with an Illumina TruSeq primer mix BR3M105 and bead clean-up BR3N401.
| Product | Fragmentation + end repair + dA-tailing | Adapter ligation reagents | Library amplification mix | Cleanup / size-selection beads | Adapters / PCR primers supplied | Default insert size | Sequencing platforms |
|---|---|---|---|---|---|---|---|
| Universal DNA Library Prep KitBR3D201 | Yes | Yes | Yes | ✕No | Not included | 150–350 bp default; ~100–1,000 bp by time | Illumina, MGI |
| Universal DNA Fragmentation ModuleBR3N602 | Yes | ✕No | ✕No | ✕No | ✕No | 200–500 bp default; ~100–1,000 bp by time | Illumina, MGI |
| AmpSeq Library Amplification MixBR3M105 | ✕No | ✕No | Yes | ✕No | Illumina (TruSeq) PCR primer mix | –Not applicable or no data | Illumina (swap primers for others) |
| NGS DNA Clean BeadsBR3N401 | ✕No | ✕No | ✕No | Yes | –Not applicable or no data | –Not applicable or no data | Platform-independent |
Pick by workflow step: T4 DNA Polymerase BR3P302 and Klenow Fragment BR3P305 blunt ends; Klenow exo– BR3P108 does fill-in and second-strand synthesis without 3′→5′ exonuclease activity; T4 PNK BR3P303 phosphorylates 5′ ends; T4 DNA Ligase BR3P301 ligates adapters; phi29 BR3P104 is the proofreading, strand-displacing enzyme for WGA and RCA.
| Product | NGS workflow step | 3′→5′ exonuclease | Strand displacement | Reaction temperature / time | Heat inactivation | Concentration |
|---|---|---|---|---|---|---|
| T4 DNA PolymeraseBR3P302 | End repair (blunting) | Yes | ✕No | 20 °C, 10 min | 72 °C, 10 min | 3 U/µL |
| Klenow FragmentBR3P305 | End repair (blunting); 5′ fill-in; Second-strand cDNA synthesis | Yes | ✕No | 37 °C, 10 min | 75 °C, 10 min | 10 U/µL |
| Klenow Fragment exo–BR3P108 | End repair (blunting); 5′ fill-in; Second-strand cDNA synthesis | ✕No | ✕No | 30–37 °C, 5–15 min | 75 °C, 10 min | 5 U/µL |
| T4 Polynucleotide KinaseBR3P303 | 5′ phosphorylation; 3′ dephosphorylation | –Not applicable or no data | –Not applicable or no data | 37 °C, 30 min | 72 °C, 10 min | 10 U/µL |
| T4 DNA LigaseBR3P301 | Adapter ligation | –Not applicable or no data | –Not applicable or no data | 23 °C, 15–20 min | 65–70 °C, 10 min | 600 U/µL |
| phi29 DNA PolymeraseBR3P104 | WGA / RCA | Yes | Yes | 30 °C, 2–8 h (isothermal) | 65 °C, 10 min | 10 U/µL |
AmpHifi HS DNA Polymerase I and III BR3P101, BR3P103 come as enzyme plus 5× buffer for purified DNA; III is faster (20 s/kb) at about 150× Taq fidelity. LongR Super-Fidelity Master Mix BR3M121 amplifies up to 30 kb from λ or plasmid templates and also works on crude bacteria, fungi, plant tissue and whole blood. For RNA templates use LongR One-Step RT-PCR BR3M321; for unextracted plant tissue, the Plant Tissue Direct kit BR1A503.
| Product | Product type | Template | Fidelity vs. Taq | Max amplicon length | Extension time | Direct PCR from crude samples | GC range |
|---|---|---|---|---|---|---|---|
| AmpHifi HS DNA Polymerase IBR3P101 | Enzyme + 5× buffer | Purified DNA | ~71× | Up to 20 kb by protocol; 10 kb gDNA demonstrated | 1 min/kb (<10 kb); 10 min total for 10–20 kb | ✕No | >70% overall, >80% local |
| AmpHifi HS DNA Polymerase IIIBR3P103 | Enzyme + 5× buffer | Purified DNA | ~150× | 20 kb (optimized buffer) | 20 s/kb | ✕No | 30–80% |
| LongR Super-Fidelity Master MixBR3M121 | 2× master mix | DNA (purified or crude) | ~154× | 30 kb (λ/plasmid), 20 kb (gDNA) | 1–20 s/kb (length-dependent) | YesBacteria, fungi, plant tissue, whole blood | 25–85% stated; 25–67% demonstrated |
| LongR One-Step RT-PCR KitBR3M321 | 2× buffer + enzyme mix (one-step RT-PCR) | RNA | ~50× | >10 kb (10.5 kb demonstrated) | 20 s–1 min/kb | ✕No | –Not applicable or no data |
| Plant Tissue Direct HiFi PCR KitBR1A503 | Lysis buffer + 2× master mix | Unextracted plant tissue | ~154× | Not stated; standard program for <2 kb, adjust for longer | 2 s/kb (<2 kb program) | YesPlant tissue | 25–67% |
Choose by sample. For sputum and BALF BR2D103 or blood, body fluids, swabs, tissue and FFPE BR2C102, both kits co-isolate DNA and RNA on magnetic beads and have an automated protocol. For total RNA from plant tissue or cultured cells, use the spin-column kit BR2B202.
| Product | Sample type | Nucleic acid isolated | Purification format | Automated extraction protocol | Sample input | Stated LOD |
|---|---|---|---|---|---|---|
| Magnetic Sputum NA Extraction KitBR2D103 | Sputum, BALF, bacterial culture | DNA + RNA (co-isolation) | Magnetic beads | Yes | 250 µL sputum | 10 copies/reaction (MTB, Flu, RSV) |
| Magnetic Microbiota DNA & RNA KitBR2C102 | Blood, body fluids, swabs, tissue, FFPE | DNA + RNA (co-isolation) | Magnetic beads | Yes | 300 µL | –Not applicable or no data |
| RNA Fast Plant Extraction KitBR2B202 | Plant tissue, cultured cells | Total RNA (gDNA removed) | Spin column | ✕No | 50–150 mg tissue | –Not applicable or no data |
Features are based on each product’s instruction manual, product page and data from our technical team. Prices, pack sizes and full specifications are on the product pages. Not every product is listed here; see the full range in NGS.
Selection guides for our other product lines.
Enzymes, qPCR, RT-qPCR and LAMP master mixes, lyoprotectants.
Selection guide Guide 02PCR, qPCR, nucleic acid extraction and protein research.
Selection guide Guide 03Residual DNA, HCP and mycoplasma QC, plus mRNA synthesis reagents.
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