T7 RNA Polymerase GMP-grade is an in-house developed and multi-step purified recombinant T7 RNA polymerase manufactured by Biori Biotech to GMP standards. It exhibits high transcriptional catalytic activity across diverse template types and nucleotide substrates.
This enzyme specifically recognizes the T7 promoter sequence (5′-TAATACGACTCACTATAG-3′) and initiates transcription from the G residue at this site, converting downstream DNA sequences into single-stranded RNA.
Using natural or modified nucleotides as substrates, and under appropriate reaction buffer conditions, T7 RNA Polymerase GMP-grade enables efficient synthesis of large quantities of RNA from DNA templates. A single 20 µL in vitro transcription reaction can yield more than 200 µg of RNA.
T7 RNA Polymerase GMP-grade, GMP-BP-E01
Features
- Manufactured to GMP standards
- In-house developed and multi-step purified recombinant T7 RNA polymerase
- High transcriptional catalytic activity across diverse template types and nucleotide substrates
- Specifically recognizes the T7 promoter sequence (5′-TAATACGACTCACTATAG-3′)
- Compatible with natural or modified nucleotides
- High-yield RNA synthesis for in vitro transcription applications
- A single 20 µL IVT reaction can yield more than 200 µg of RNA
Application
- In vitro transcription
- RNA synthesis from T7 promoter-containing DNA templates
- RNA production using natural nucleotides
- RNA production using modified nucleotides
Components
| No. | Component | Catalog No. | Quantity | Volume |
|---|---|---|---|---|
| 1 | T7 RNA Polymerase GMP-grade (50 U/µL) | GMP-BP-E01-5K | 5 KU | 100 µL |
| 2 | T7 RNA Polymerase GMP-grade (50 U/µL) | GMP-BP-E01-50K | 50 KU | 1 mL |
| 3 | T7 RNA Polymerase GMP-grade (50 U/µL) | GMP-BP-E01-500K | 500 KU | 10 mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20 ± 5 °C. |
| Handling | Vortex before use. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% (v/v) Triton X-100, 50% (v/v) glycerol, pH 7.9 at 25°C. |
Data
Coming Soon…
