Home » Product » Life Science » Molecular Biology » Utility Enzymes » Restriction Endonucleases » Aci I, BR1G102
Product Catalog
Aci I is a Type IIP restriction endonuclease expressed in Escherichia coli carrying the Aci I gene cloned from Arthrobacter citreus. It specifically recognizes and cleaves the sequence 5’…C↓CGC…3′ / 3’…GGC↑G…5′.
Supplied with 10× Cut-Buffer, Aci I is suitable for routine DNA digestion workflows involving genomic DNA, plasmid DNA, and PCR products. This enzyme is optimized for incubation at 37°C and provides reliable restriction digestion performance for molecular biology research applications.
Biori Aci I is designed for research use in restriction enzyme digestion workflows requiring sequence-specific DNA cleavage and convenient heat inactivation after digestion.
Features
- Type IIP restriction endonuclease recognizing 5’…C↓CGC…3′ / 3’…GGC↑G…5′.
- Expressed in Escherichia coli carrying the Aci I gene cloned from Arthrobacter citreus.
- Supplied at 10 U/µL with 10× Cut-Buffer for convenient reaction setup.
- Suitable for digestion of genomic DNA, plasmid DNA, and PCR products.
- Optimized for incubation at 37°C for 1 h.
- Heat inactivation supported at 65°C for 20 min.
Application
- Restriction digestion of genomic DNA
- Restriction digestion of plasmid DNA
- Restriction digestion of PCR products
- Sequence-specific DNA cleavage in molecular biology workflows
Components
| Component | BR1G102-01 | BR1G102-02 | BR1G102-03 |
|---|---|---|---|
| Aci I (10 U/µL) | 1 × 0.01 mL | 1 × 0.02 mL | 1 × 0.1 mL |
| 10× Cut-Buffer | 1 × 1 mL | 1 × 1 mL | 1 × 1 mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship with ice packs. |
| Handling | Mix thoroughly before use. Avoid repeated freeze-thaw cycles. |
| Enzyme Input | The volume of restriction endonuclease added should not exceed one-tenth of the total reaction volume. |
| Reaction Condition | Incubate at 37°C for 1 h. |
| Heat Inactivation | Incubate at 65°C for 20 min. |
| Handling Note | Digestion for longer than 3 h is not recommended, as prolonged incubation may lead to star activity. |
| Intended Use | For research use only. Not for use in clinical diagnosis. |
Data
Coming Soon…
