AcuGenix™ Hot Start Taq DNA Polymerase (Glycerol-Free) is a chemically modified hot-start Taq DNA polymerase optimized and formulated specifically for the special requirements of freeze-dried reagents. It completely blocks Taq enzyme activity at room temperature and effectively suppresses non-specific amplification caused by primer annealing or primer dimerization at low temperatures, thereby improving the specificity and sensitivity of PCR reactions. It can also work in a “time release” mode to gradually release enzyme activity during PCR cycling, further enhancing amplification specificity and sensitivity for low-copy templates. In fluorescent PCR applications, it offers high sensitivity, high fluorescence intensity, and high specificity.
| No. | Component | Notes |
|---|---|---|
| 1 | 5 U/μL AcuGenix™ Hot Start Taq DNA Polymerase (Glycerol-Free) | — |
| 2 | 10× HS Buffer (Mg2+-free) | Optional |
| 3 | 25 mM MgCl2 | Optional |
Note: 10× HS Buffer (Mg2+-free) does not contain dNTPs or Mg2+. Please add dNTPs and MgCl2 when preparing reaction systems.
| Condition | Recommendation |
|---|---|
| Storage | Store at −20 ± 5°C. |
| Handling | Mix thoroughly before use and avoid repeated freeze–thaw cycles. |
| Storage Buffer | 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer. |
Coming Soon…
* Your data is securely protected for essential communications only. We won’t share your details or send spam emails, Privacy Policy *
