Additional information
| Size | 250U, 500U |
|---|
FEN1, BR1G101
Price range: $20.00 through $35.00
FEN1 is a thermostable nuclease derived from the FEN1 gene of Thermococcus 9°N. It specifically recognizes and cleaves 5′ flap DNA structures in branched double-stranded DNA substrates, generating ligatable 5′-phosphate ends for downstream DNA ligation.
The cleavage products generated by FEN1 can be efficiently ligated by DNA ligase to form double-stranded DNA, making this enzyme useful for structure-specific DNA processing workflows. In vivo, FEN1 plays an essential role in Okazaki fragment maturation and also participates in base excision repair.
Supplied with a dedicated 10 × reaction buffer, Biori FEN1 is suitable for research applications involving 5′ flap DNA cleavage and structure-specific DNA analysis.
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