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FEN1 is a thermostable nuclease derived from the FEN1 gene of Thermococcus 9°N. It specifically recognizes and cleaves 5′ flap DNA structures in branched double-stranded DNA substrates, generating ligatable 5′-phosphate ends for downstream DNA ligation.
The cleavage products generated by FEN1 can be efficiently ligated by DNA ligase to form double-stranded DNA, making this enzyme useful for structure-specific DNA processing workflows. In vivo, FEN1 plays an essential role in Okazaki fragment maturation and also participates in base excision repair.
Supplied with a dedicated 10 × reaction buffer, Biori FEN1 is suitable for research applications involving 5′ flap DNA cleavage and structure-specific DNA analysis.
Features
- Derived from the FEN1 gene of Thermococcus 9°N.
- Specifically recognizes and cleaves 5′ flap DNA structures in branched double-stranded DNA.
- Generates ligatable 5′-phosphate ends for downstream DNA ligation.
- Supplied at 32 U/µL with a dedicated 10 × FEN1 Reaction Buffer.
- Optimized for incubation at 65°C with a rapid 15 min reaction protocol.
- Applicable to structure-specific DNA cleavage workflows in molecular biology research.
Application
- Cleavage of structure-specific DNA
- 5′ flap DNA substrate processing
- DNA ligation workflow support after flap cleavage
- Okazaki fragment maturation research
- Base excision repair research
Components
| Component | BR1G101-01 | BR1G101-02 |
|---|---|---|
| FEN1 (32 U/µL) | 1 × 0.05 mL | 1 × 0.1 mL |
| 10 × FEN1 Reaction Buffer | 1 × 1 mL | 1 × 1 mL |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Handling | Mix thoroughly before use. Avoid repeated freeze-thaw cycles. |
| Enzyme Input | The volume of nuclease added should not exceed one-tenth of the total reaction volume. |
| Reaction Condition | Incubate at 65°C for 15 min. |
| Handling Note | Prolonged incubation is not recommended, as extended digestion may cause DNA deamination. |
| Intended Use | For research use only. Not for use in clinical diagnosis. |
Data
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