SuperStart Taq Plus DNA Polymerase is a novel hot-start DNA polymerase developed by Biori. The enzyme utilizes antibody-based hot-start modification to effectively suppress non-specific amplification caused by primer misannealing or primer-dimer formation during PCR setup and amplification.
This enzyme demonstrates excellent performance in multiplex PCR applications and provides optimized amplification efficiency for extremely low-concentration DNA templates. It enables higher product yields, improved specificity, and stable amplification results, making it suitable for both conventional PCR and quantitative PCR assays.
| No. | Component | Notes |
|---|---|---|
| 1 | 5 U/µL SuperStart Taq Plus DNA Polymerase | — |
| 2 | 10× PCR Buffer II (Mg²⁺-free) | Optional |
| 3 | 25 mM MgCl₂ | Optional |
Note: The 10× PCR Buffer II (Mg²⁺-free) does not contain dNTPs or Mg²⁺. Please add dNTPs and MgCl₂ when preparing the PCR reaction system.
| Condition | Recommendation |
|---|---|
| Shipping | Ship on dry ice. |
| Storage | Store at −20 ± 5°C. |
| Handling | Mix thoroughly before use and avoid repeated freeze–thaw cycles. |
Experimental validation data including quantitative gradient tests, real-time amplification comparison, and accelerated stability testing will be updated soon.
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