5× SDS-PAGE Protein Loading Buffer
5× SDS-PAGE Protein Loading Buffer is a 5× concentrated protein sample loading buffer.
Product overview
5× SDS-PAGE Protein Loading Buffer is a 5× concentrated protein sample loading buffer. SDS binds to proteins and confers a strong negative charge on protein-SDS complexes, thereby masking the intrinsic charge differences among proteins. At the same time, SDS disrupts intra- and intermolecular hydrogen bonds and denatures the secondary and tertiary structures of proteins. The reducing agent in the loading buffer also cleaves disulfide bonds between cysteine residues and disrupts protein conformation. As a result, structural differences among proteins are minimized, and migration of protein bands during electrophoresis depends mainly on molecular weight.
Features and applications
- This product is a denaturing and reducing protein loading buffer formulated with an updated composition that helps maintain a neutral pH after sample boiling, thereby reducing the risk of protein degradation.
- The new-generation reducing agent provides strong reducing performance while avoiding the odor associated with β-mercaptoethanol or DTT.
- During sample loading, the sample settles quickly and is less likely to float out of the wells.
- After storage at -20°C, the product thaws rapidly to reduce waiting time.
- Protein loading buffer for denaturing and reducing SDS-PAGE (not suitable for electrophoresis under native conditions).
Components
| Components | BR4C351-01 | BR4C351-02 | BR4C351-03 |
|---|---|---|---|
| 5× SDS-PAGE Protein Loading Buffer | 5 mL | 15 mL | 75 mL |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Shipped with ice packs. |
| Handling | For research use only. Review the MSDS before use. |
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