180 kDa Prestained Protein Marker

180 kDa Prestained Protein Marker consists of 10 prestained proteins in three colors with molecular weights in the range of 13-180 kDa.

13–180 kDa

Range indicated

3 colors

Orange-red, green and blue bands

50 freeze-thaw cycles

Stable 3 months at 4 °C

Product overview

180 kDa Prestained Protein Marker consists of 10 prestained proteins in three colors with molecular weights in the range of 13-180 kDa. The 75 kDa band is orange-red, the 25 kDa band is green, and the 13 kDa, 17 kDa, 36 kDa, 45 kDa, 60 kDa, 100 kDa, 140 kDa, and 180 kDa bands are blue. This product is intended for SDS-PAGE and clearly indicates electrophoresis progress while providing a useful reference for estimating target-protein molecular weight. In Western blot experiments, it can be used to monitor transfer performance on PVDF or NC membranes and to help assess target-protein detection. This product is compatible with Tris-Glycine gels (Tris-Glycine running buffer) and Bis-Tris gels (MOPS or MES running buffer). All protein bands have been calibrated against unstained protein markers to ensure accurate band sizes. The recommended loading volume is 5 μL per lane.

Features and applications

Features

Clear bands: All bands are clearly visible and provide a clear indication of electrophoresis progression.
Three-color design: Features orange-red, green, and blue reference bands for easy identification.
Wide indication range: Molecular weight range of 13–180 kDa.

Applications

Indicates electrophoresis progress.
Verifies Western blot transfer efficiency.
Pre-stained in three colors, with a molecular weight range of 13–180 kDa.

Components

Components	BR4C303-01 100 T	BR4C303-02 500 T
180 kDa Prestained Protein Marker	2×0.25 mL	10×0.25 mL

Performance data

Schematic diagram of protein bands.

BR4C303 180 kDa Prestained Protein Marker: Figure 1. Schematic diagram of BR4C303 bands, SDS-PAGE gel, and PVDF membrane transfer efficiency. BR4C303 can clearly indicate the electr — Figure 1 Schematic diagram of BR4C303 bands, SDS-PAGE gel, and PVDF membrane transfer efficiency. BR4C303 can clearly indicate the electrophoresis progress and allow relatively accurate determination of the molecular weight of the target protein.

Migration pattern diagram.

BR4C303 180 kDa Prestained Protein Marker: Figure 2. Migration patterns of BR4C303 under different electrophoresis conditions. — Figure 2 Migration patterns of BR4C303 under different electrophoresis conditions.

This product is compatible with Tris-Glycine gels (Tris-Glycine running buffer) and Bis-Tris gels (MOPS or MES running buffer). The optimal separation range varies depending on the gel percentage and the running buffer system used (e.g., 6-15% for Tris-Glycine, 4-12% for Bis-Tris, with MES showing a lower molecular weight separation range than MOPS).