NGS Enzymes for Library Construction
Eight enzymes for end repair, A-tailing, ligation, fill-in and library amplification: hot-start high-fidelity polymerases, Klenow fragments, T4 DNA ligase, T4 PNK and phi29.
Pick the enzyme by library-prep step
Start from what you need to do. Each row names the product we would pick first and the alternatives in the same group; the full list is below.
| Use case | Recommended product | Why this one | Alternatives |
|---|---|---|---|
| High-fidelity amplification | AcuGenix™ AmpHifi HS DNA Polymerase I BR3P101 Data | Antibody hot-start high-fidelity polymerase with 3′→5′ proofreading for low-error library amplification, including long and GC-rich templates | BR3P103 |
| Strand-displacement amplification | AcuGenix™ phi29 DNA Polymerase BR3P104 Data | phi29 DNA polymerase with strand displacement and >70 kb processivity for isothermal whole-genome and rolling-circle amplification | — |
| End repair and fill-in | T4 DNA Polymerase BR3P302 Data | T4 DNA polymerase with strong 3′→5′ proofreading and no 5′→3′ exonuclease, converting fragmented DNA to blunt ends before adapter ligation | BR3P305, BR3P108 |
| 5′ phosphorylation | T4 Polynucleotide Kinase (T4 PNK) BR3P303 Data | Versatile End-Modification Enzyme for NGS, Probe Labeling & Molecular Cloning | — |
| Ligation | T4 DNA Ligase BR3P301 Data | T4 DNA ligase with high blunt-end efficiency and no detectable exonuclease activity for adapter ligation in amplicon and shotgun libraries | — |
8 products
AcuGenix™ AmpHifi HS DNA Polymerase I
High-Fidelity Hot-Start Polymerase for Specific, Robust PCR Workflows
AcuGenix™ AmpHifi HS DNA Polymerase III
Next-Generation Hot-Start High-Fidelity Polymerase for Demanding PCR & NGS Workflows
AcuGenix™ phi29 DNA Polymerase
High-Fidelity, Long-Range Isothermal DNA Polymerase for Advanced Genomic Workflows
T4 DNA Polymerase
Template-Dependent Polymerase with Robust Proofreading Activity for Precision Nucleic Acid Manipulation
Klenow Fragment (DNA Polymerase I Large Fragment)
High-Fidelity Polymerase with Optimized Exonuclease Profile for Precision Molecular Workflows
Klenow Fragment (3’→5’ exo-)
Exonuclease-Deficient Mutant for 3’-End-Preserving DNA Synthesis | Ideal for NGS Library Prep & Probe Labeling
T4 Polynucleotide Kinase (T4 PNK)
Versatile End-Modification Enzyme for NGS, Probe Labeling & Molecular Cloning
T4 DNA Ligase
High-Efficiency ATP-Dependent Ligation for Cloning, NGS & Molecular Biology Workflows
Modules built on these enzymes
Universal DNA Fragmentation Module BR3N602, AmpSeq Library Amplification Mix BR3M105, Universal DNA Library Prep Kit BR3D201.
Go to NGS Modules →Documents
Manuals, COA and SDS are linked on each product row; the NGS Library Series catalog collects them.
Download catalog (PDF) →Custom and OEM
Bulk fills, glycerol-free or custom-buffer formats and pilot evaluation are quoted per project.
Talk to an expert →Frequently asked questions
Which products are in the NGS Enzyme category?
What documents are provided?
Can I order bulk packs or a custom formulation?
Need the whole enzyme set for a kit?
Send your workflow; we propose the set and evaluation quantities.
