Bacteria DNA Detection Kit (qPCR), BP-QN99, is a fluorescent probe qPCR kit for qualitative detection of bacterial DNA contamination in biological samples. The assay targets conserved bacterial 16S rRNA coding regions and includes an internal control for workflow monitoring.
Bacteria DNA Detection Kit (qPCR), BP-QN99
Product information published by Zhuhai Biori Biotechnology Co., Ltd.
Features
- qPCR probe-based assay for conserved bacterial 16S rRNA coding sequences.
- Internal control can be used to evaluate extraction efficiency or monitor amplification inhibition.
- Contains UDG enzyme to help prevent contamination from amplified products.
- Manual states a detection limit of 10 CFU/reaction when used with the specified Biori extraction or purification kit.
- COA reports conforming negative concordance, positive concordance, LOD, and internal-standard Ct results.
Application
- Qualitative bacteria DNA contamination testing.
- Testing for cells, cell and gene therapy samples, and other biological products.
- qPCR workflows using supported real-time PCR systems listed in the manual.
- Sterility and microbial safety testing workflows paired with nucleic acid extraction or purification.
Components
| Component | Specification |
|---|---|
| Bac qPCR MIX | 1.0 mL x 1 tube |
| Bac Positive Control | 1.5 mL x 2 tubes |
| Bac Negative Control | 1.5 mL x 2 tubes |
| Bac Internal Control | 1.0 mL x 1 tube |
| Instructions | 1 copy |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Storage | <= -18 C, protected from light. Shelf life: 24 months. Avoid more than 7 freeze-thaw cycles. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Protect from light and avoid unnecessary freeze-thaw cycles where applicable. |
Data
Performance Data
Bacteria DNA Detection Kit (qPCR) | Catalog No. BP-QN99-50
A product-specific validation study evaluated qPCR sensitivity across nine bacterial strains, cross-reactivity with non-target materials, and performance across common sample matrices.
Key Performance at a Glance
| Perf |
Sele |
|---|---|
| Eval |
100 CFU per 200 μL samp |
| Eval |
9 stra |
| Cros |
11 non- |
| Matr |
8 sour |
Analytical Sensitivity
Observed in this validation study: at 100 CFU per 200 μL sample, corresponding to 10 CFU/reaction, each of the nine evaluated bacterial strains produced 24 positive results in 24 replicates.
| Orga |
Dete |
|---|---|
| Stap |
24/ |
| Pseu |
24/ |
| Baci |
24/ |
| Clos |
24/ |
| Micr |
24/ |
| Salm |
24/ |
| Esch |
24/ |
| Cuti |
24/ |
| Stre |
24/ |
Cross-Reactivity
Observed in this validation study: all three replicates were negative for each of the 11 evaluated non-target materials.
| Samp |
Repl |
Repl |
Repl |
|---|---|---|---|
| MDBK | Nega |
Nega |
Nega |
| PK15 | Nega |
Nega |
Nega |
| CHO | Nega |
Nega |
Nega |
| HEK2 |
Nega |
Nega |
Nega |
| C6 | Nega |
Nega |
Nega |
| CRFK | Nega |
Nega |
Nega |
| SF9 | Nega |
Nega |
Nega |
| Huma |
Nega |
Nega |
Nega |
| Cand |
Nega |
Nega |
Nega |
| Aspe |
Nega |
Nega |
Nega |
| Myco |
Nega |
Nega |
Nega |
Matrix Interference
The assay generated comparable results across the source-listed matrices under the evaluated study conditions.
| No. | Matr |
Resu |
Resu |
Resu |
|---|---|---|---|---|
| 1 | DMEM | 31.18 | 30.85 | 30.25 |
| 2 | RPMI 1640 medi |
30.05 | 30.08 | 30.60 |
| 3 | 20% FBS | 29.73 | 29.67 | 30.02 |
| 4 | PBS | 29.68 | 30.55 | 30.72 |
| 5 | Feta |
29.01 | 29.24 | 29.88 |
| 6 | Tryp |
29.62 | 29.94 | 30.35 |
| 7 | Sabo |
28.86 | 29.29 | 29.81 |
| 8 | Norm |
29.06 | 29.40 | 30.46 |
| 9 | Cont |
30.59 | 29.88 | 30.06 |
Study Scope and Limitations
Results apply to the nine evaluated bacterial strains, non-target panel, matrices, and study conditions. This selected upload version does not make a universal bacterial coverage claim. Users should verify suitability with their own organisms, sample matrix, and workflow.
