EndoFree Plasmid Maxi Kit

The EndoFree Plasmid Maxi Kit utilizes a unique silica membrane adsorption technology to bind plasmid DNA with high efficiency and specificity.

200 µg–1.5 mg

Plasmid DNA in about 1 h

Endotoxin-free

Transfection-grade

High supercoil ratio

Better transfection than Supplier N

Product overview

The EndoFree Plasmid Maxi Kit utilizes a unique silica membrane adsorption technology to bind plasmid DNA with high efficiency and specificity. The extracted plasmid is of high purity and low endotoxin content, with high recovery. The whole experimental procedure of plasmid DNA extraction could be finished within 1 h. Plasmids purified with this kit can be used for various routine procedures, including Restriction Digestion, PCR, Sequencing, Ligation, Transformation, Gene Therapy, Cell Microinjection, Gene Silencing, and Cell Transfection experiments.

Features and applications

Features

Rapid & High Yield: It allows isolation of 200 μg–1.5 mg high-quality plasmid DNA within approximately 1 hour.
High Plasmid Purity: The isolated plasmids exhibit a high supercoiled-form ratio.
Superior Transfection Performance: Suitable for advanced transfection experiments with most cell lines.

Applications

EndoFree Plasmid Maxi Kit isolation yields DNA suitable for routine experiments including Restriction Digestion, PCR, Sequencing, Ligation, and Transformation, as well as advanced applications such as Gene Therapy, Cell Microinjection, Gene Silencing, and Cell Transfection.

Components

Component	BR2D102-00 2 T	BR2D102-01 10 T
Buffer BL	10 mL	30 mL
Buffer E1	30 mL	100 mL
Buffer E2	30 mL	100 mL
Buffer E3	30 mL	100 mL
Buffer PW	15 mL	70 mL
Buffer TB	5 mL	30 mL
RNase A (10 mg/mL)	0.35 mL	1.2 mL
Filtration CS1	2	10
Spin Columns CP1	2	10
Collection Tubes 50 mL	2	10

Performance data

Plasmid extraction yield

Plasmid Quality and Purity

Enables highly efficient cell transfection.

Shipping and storage

Condition	Recommendation
Storage	Store at room temperature (15-30°C). Before the first use, add all RNase A to Buffer E1, mix well, and store at 2-8°C.
Shipping	Refer to product label and shipping documentation.