N1-Me-pUTP Solution GMP-grade (100 mM)
N1-methylpseudouridine triphosphate for modified RNA synthesis.
Product overview
N1-Me-pUTP is one of the most commonly used modified nucleoside triphosphates. It introduces methylation to the pseudouridine structure, a structural analog modification that alters the conformation and immunogenicity of RNA. This modification changes the linkage between the ribose and uracil from a C-N bond to a C-C bond and adds a methyl group at the N1 position of the base. This modification effectively evades recognition by immune sensors (such as TLR3, TLR7, etc.), significantly reducing the innate immune response; it also resists nuclease degradation and enhances ribosome binding to mRNA. It is primarily applied in research fields such as mRNA vaccines, gene therapy, protein replacement therapy, and cell reprogramming. This product is manufactured in accordance with GMP requirements.
Features and applications
- GMP-grade nucleotide solution for RNA synthesis workflows.
- Concentration: 100 mM±3 mM; purity: ≥99% (by HPLC).
- Designed for in vitro transcription of mRNA and related molecular biology applications.
- Nucleotide substrate for in vitro transcription.
- Research on mRNA vaccines, gene therapy, protein replacement therapy, and cell reprogramming.
Components
| Item | Specification |
|---|---|
| Molecular Weight | 564.11 |
| CAS | 1428903-59-6 (free acid) |
| Molecular Formula | C10H14N2Na3O15P3 |
| Concentration | 100mM±3mM |
| Purity | ≥99%(by HPLC) |
| max λ | 272nm±2nm |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Storage | Store at -20±5°C. |
| Shipping | Ship frozen on dry ice or with validated frozen cold-chain packaging that maintains the specified frozen storage condition. |
| Handling | Avoid repeated freeze-thaw cycles; mix thoroughly after thawing where applicable. |
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