Plasmid Miniprep Kit

The Plasmid Miniprep Kit uses the alkaline lysis method to lyse cells.

> 85% recovery

Plasmid DNA from culture

Product overview

The Plasmid Miniprep Kit uses the alkaline lysis method to lyse cells. It employs a unique silica membrane spin column that specifically binds plasmid DNA under high-salt and low-pH conditions, removing proteins, RNA, genomic DNA, and other impurities to yield high-quality, high-purity plasmid DNA. Each spin column can bind up to 30 µg of plasmid DNA, making it suitable for the efficient extraction of plasmid DNA from 1–5 mL of overnight-cultured Escherichia coli. The yield and quality of the extracted plasmid are affected by factors such as host strain type and culture conditions, cell lysis efficiency, plasmid copy number, and plasmid stability. Plasmid DNA extracted using this kit can be directly used in various routine molecular biology experiments, including restriction digestion, ligation, PCR, sequencing, and transformation.

Features and applications

Features

Fast: Fewer steps, simple operation, and time-saving.
Efficient: High plasmid yields from both high- and low-copy plasmids (see Figure 1).

Applications

Plasmid minipreparation for molecular biology experiments such as enzyme digestion, transformation, sequencing, and PCR.

Components

Components	BR2D101-01 50T	BR2D101-02 200T
Buffer P1	15 mL	60 mL
Buffer P2	15 mL	60 mL
Buffer P3	20 mL	80 mL
Buffer PD	30 mL	120 mL
Buffer PW	15 mL	50 mL
Elution Buffer	15 mL	30 mL
Spin Columns with Collection Tubes	50 sets	200 sets
RNase A (10 mg/mL)	150 µL	600 µL

Performance data

Plasmid extraction efficiency test results

Note: Plasmids with different copy numbers were extracted using Biori BR2D101, and the plasmid concentrations were measured by NanoDrop. The results showed that this kit exhibited good extraction performance and high yields for plasmids with various copy numbers.

Quality verification results of extracted plasmid

Note: The pUC57, pRSFDuet-1, and pcDNA3.1 plasmids extracted as described above were each digested with two restriction enzymes and examined by agarose gel electrophoresis. The results indicated that the purified plasmids were of high quality and exhibited no inhibitory effect on subsequent enzyme digestion.

Shipping and storage

Condition	Recommendation
Storage	The kit components can be stored dry at room temperature (15–30°C) for up to 18 months. If precipitation occurs in the solutions, preheat in a 37°C water bath for 10 min before use to dissolve the precipitate, which does not affect performance. RNase A is stable for 18 months when stored at −20±5°C. Prior to first use, add all RNase A into Buffer P1, mix well, and store at 2–8°C for stable storage up to 6 months.
Shipping	Shipped at ambient temperature.

Condition

Recommendation

Storage

The kit components can be stored dry at room temperature (15–30°C) for up to 18 months. If precipitation occurs in the solutions, preheat in a 37°C water bath for 10 min before use to dissolve the precipitate, which does not affect performance. RNase A is stable for 18 months when stored at −20±5°C. Prior to first use, add all RNase A into Buffer P1, mix well, and store at 2–8°C for stable storage up to 6 months.

Shipping

Shipped at ambient temperature.