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Spiroplasma DNA Detection Kit (qPCR), BP-QN299, provides qualitative detection of Spiroplasma contamination in biopharmaceutical materials using probe-based qPCR with an internal control.

Spiroplasma DNA Detection Kit (qPCR), BP-QN299

USD

$0.00

BP-QN299-50

Size

- 100T - $1180.00

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Product information published by Zhuhai Biori Biotechnology Co., Ltd.

Features

Probe-based qPCR assay targeting conserved Spiroplasma 16S rRNA regions.
Internal control supports extraction-efficiency evaluation and amplification-inhibition monitoring.
UDG enzyme helps prevent amplification-product carryover contamination.
Manual-stated detection limit: up to 10 CFU/mL when used with BIORI nucleic acid extraction or purification reagent kits.
50 reactions per kit.

Application

Qualitative detection of Spiroplasma contamination in production raw materials and cell banks.
Testing of viral seeds and viral or cellular harvest solutions.
Testing of therapeutic cells in research-use workflows.

Components

Component	Specification (50 Reactions)
Spi qPCR MIX	1.0 mL × 1 tube
Spi Positive Control	1.5 mL × 2 tubes
Spi Negative Control	1.5 mL × 2 tubes
Spi Internal Control	1.0 mL × 1 tube
Instructions	1 copy

Shipping and Storage

Condition	Recommendation
Shipping Temperature	Ship frozen on dry ice or with validated frozen cold-chain packaging.
Storage Temperature	≤ -18°C, protected from light.
Shelf Life	24 months. Avoid more than 7 freeze-thaw cycles.

Data

Performance Data

Spiroplasma DNA Detection Kit (qPCR) | Catalog No. BP-QN299-50

A product-specific validation study evaluated analytical sensitivity for Spiroplasma citri, cross-reactivity with non-target DNA materials, and performance across common matrices.

Key Performance at a Glance

Selected performance summary for BP-QN299-50
Performance parameter	Selected result
Limit of detection	10 CFU/mL for S. citri
Cross-reactivity panel	14 non-target DNA materials
Matrix panel	6 source-listed matrices

Analytical Sensitivity

Observed in this validation study: at 10 CFU/mL, Spiroplasma citri was detected in 24 of 24 replicates.

Analytical-sensitivity result (source Table 1)
Strain	Concentration (CFU/mL)	Detection rate
Spiroplasma citri	10	24/24 detections (100%)

Cross-Reactivity

Observed in this validation study: both replicates were negative for each of the 14 evaluated non-target DNA materials.

Cross-reactivity results (source Table 2)
No.	Sample	Replicate 1	Replicate 2
1	Hi5	Negative	Negative
2	Sf9	Negative	Negative
3	Clostridium sporogenes	Negative	Negative
4	SP2/0	Negative	Negative
5	C6	Negative	Negative
6	E. coli	Negative	Negative
7	Pseudomonas aeruginosa	Negative	Negative
8	Mycoplasma orale	Negative	Negative
9	Mycoplasma pneumoniae	Negative	Negative
10	Micrococcus sp.	Negative	Negative
11	Cutibacterium acnes	Negative	Negative
12	Streptococcus pyogenes	Negative	Negative
13	HeLa	Negative	Negative
14	Vero	Negative	Negative

Matrix Interference

The selected tables retain results for spiked matrices and their corresponding unspiked matrix controls.

Results for Spiroplasma-spiked matrices (source Table 3)
No.	Matrix / medium	Result 1	Result 2
1	RPMI 1640 + Spiroplasma	30.41	30.33
2	DMEM + Spiroplasma	30.34	30.37
3	Mycoplasma broth + Spiroplasma	30.11	29.76
4	DMEM (20% FBS) + Spiroplasma	29.61	29.32
5	PBS + Spiroplasma	30.25	30.35
6	DMEM (20% FBS + 10% DMSO) + Spiroplasma	30.00	29.91
7	Kit negative control + Spiroplasma	29.90	29.59

Results for unspiked matrix controls (source Table 4)
No.	Matrix / medium	Result 1	Result 2
1	1640	Negative	Negative
2	DMEM	Negative	Negative
3	Mycoplasma broth	Negative	Negative
4	DMEM (20% FBS)	Negative	Negative
5	PBS	Negative	Negative
6	DMEM (20% FBS + 10% DMSO)	Negative	Negative

Study Scope and Limitations

Results apply to S. citri, the evaluated non-target panel, matrices, and study conditions. This selected upload version does not make a universal Spiroplasma coverage claim. Users should verify suitability with their own organism, sample matrix, and workflow.

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