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RNase H II

RNase H II

  • Item number:E201
  • Overview
  • experimental

Ribonuclease H II (RNase H II) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA on DNA/RNA hybrid strands, but does not hydrolyzes phosphodiester bonds in single/double stranded DNA or RNA. The enzyme works by breaking the phosphodiester linking RNA bases in 5' direction to DNA bases, generating a 3' hydroxyl end and a phosphate group at the 5' end of the ribonucleic acid. 


Reagent Composition:

RNase H Ⅱ 200mU/μL


Application:

It can be used in PCR amplification system, qPCR, LAMP and other reaction systems that rely on endoribonuclease H.

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