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Product Catalog
AcuGenix™ DNase I (Deoxyribonuclease I, abbreviated as DNase I) is a deoxyribonuclease I produced through recombinant expression in a Pichia pastoris strain. This enzyme efficiently digests single-stranded or double-stranded DNA fragments.
Features
- AcuGenix™ DNase I (Deoxyribonuclease I, abbreviated as DNase I) is a deoxyribonuclease I produced through recombinant expression in a Pichia pastoris strain. This enzyme efficiently digests single-stranded or double-stranded DNA fragments. The activity of DNase I is highly dependent on Ca2+ levels and can be activated by Mg2+ or Mn2+.
Application
- Used in RNA extraction experiments to prepare DNA-free RNA samples. 2. Used in in vitro transcription to linearize plasmid templates. 3. Used to degrade residual genomic DNA prior to RT-PCR experiments. 4. Used in footprinting assays to analyze interactions between DNA and proteins. 5. Used to prepare DNA libraries containing random fragments. 6. Used in conjunction with DNA Polymerase I for DNA labeling via the gap-shift method. 7. Used to partially cleave genomic DNA as a positive control in TUNEL assays for apoptosis detection.
Components
| Component | Specification / Notes |
|---|---|
| 2 U/μL DNase I | 2U/μL |
| 10×DNase I Reaction Buffer (optional) | Included component; refer to the instruction manual |
Shipping and Storage
| Condition | Recommendation |
|---|---|
| Shipping Temperature | Ice pack transportation（2-8℃） |
| Storage Temperature | -20±5℃ |
| Shelf Life | 18M |
Data
Coming soon.
