Nuclease · Cat. no. E204
AcuGenix™ DNase I
AcuGenix™ DNase I, E204, is supplied for use in RNA extraction experiments to prepare DNA-free RNA samples.
Pichia-expressed
Recombinant DNase I
ss + ds DNA
Digests both substrates
Product overview
AcuGenix™ DNase I (Deoxyribonuclease I, abbreviated as DNase I) is a deoxyribonuclease I produced through recombinant expression in a Pichia pastoris strain. This enzyme efficiently digests single-stranded or double-stranded DNA fragments.
Features and applications
Features
- AcuGenix™ DNase I (Deoxyribonuclease I, abbreviated as DNase I) is a deoxyribonuclease I produced through recombinant expression in a Pichia pastoris strain. This enzyme efficiently digests single-stranded or double-stranded DNA fragments. The activity of DNase I is highly dependent on Ca2+ levels and can be activated by Mg2+ or Mn2+.
Applications
- Used in RNA extraction experiments to prepare DNA-free RNA samples.
- Used in in vitro transcription to linearize plasmid templates.
- Used to degrade residual genomic DNA prior to RT-PCR experiments.
- Used in footprinting assays to analyze interactions between DNA and proteins.
- Used to prepare DNA libraries containing random fragments.
- Used in conjunction with DNA Polymerase I for DNA labeling via the gap-shift method.
- Used to partially cleave genomic DNA as a positive control in TUNEL assays for apoptosis detection.
Components
| Component | Specification / Notes |
|---|---|
| 2 U/μL DNase I | 2U/μL |
| 10×DNase I Reaction Buffer (optional) | Included component; refer to the instruction manual |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Temperature | Ice pack transportation (2-8°C) |
| Storage Temperature | -20±5°C |
| Shelf Life | 18M |
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