Restriction Endonucleases
Fast-cut restriction endonucleases that complete site-specific DNA digestion within 5-15 min and retain 100% activity in the universal 10× Cut-Buffer.
Pick the enzyme by end type and site
All fast-cut enzymes retain 100% activity in the universal 10× Cut-Buffer, so any two can be combined without buffer exchange. Each row names a first pick for one type of end or site and lists the other enzymes of the same type; the full recognition-site table follows the product list.
| Use case | Recommended product | Why this one | Alternatives |
|---|---|---|---|
| Sticky ends: 5′ overhang, 6-bp site | EcoRI BR1G111 Data | G↓AATTC with a 4-base 5′ overhang; heat-inactivated at 80°C for 20 min. | AflII, ApaLI, AvrII, BamHI, BclI, BglII, BsaHI, BsiWI, BspEI, BsrFI, BssHII, ClaI, HindIII, KasI, MluI, MunI, NcoI, NdeI, SalI, XbaI, XhoI |
| Sticky ends: 3′ overhang, 6-bp site | PstI BR1G147 Data | CTGCA↓G with a 4-base 3′ overhang; not heat-inactivatable (purify by column or phenol-chloroform). | AatII, EcoT22I, KpnI, SacII |
| Blunt ends, 6-bp site | EcoRV BR1G149 Data | GAT↓ATC, blunt ends; heat-inactivated at 80°C for 20 min. | DraI, FspI, HpaI, MscI, NaeI, NruI, PmlI, PvuII, ScaI, SmaI, SnaBI, SspI |
| Rare cutters, 8-bp site | AscI BR1G131 Data | 8-bp site GG↓CGCGCC with a 4-base 5′ overhang; heat-inactivated at 80°C for 20 min. | PmeI, SdaI |
| Type IIS: cuts outside the site | BsaI BR1G110 Data | Cuts outside its GGTCTC site, GGTCTC(N1)↓ on the top strand and (N5)↑ on the bottom strand, leaving a 4-base 5′ overhang. | BspQI, FokI |
| GATC: methylated or unmethylated DNA | DpnI BR1G122 Data | Recognises the Dam-methylated site GAᵐ⁶↓TC and leaves blunt ends; Dam methylation blocks cleavage by DpnII and MboI at the same GATC site. | DpnII, MboI |
| Frequent cutters, 4- and 5-bp site | HaeIII BR1G141 Data | 4-bp GG↓CC, blunt ends; the manual lists no effect of Dam, Dcm, CpG, EcoKI or EcoBI methylation. | AciI, AluI, HinfI, HinP1I, HpaII, PspGI, RsaI, TaqI |
| Interrupted sites | BstEII BR1G130 Data | 7-bp interrupted site G↓GTNACC with a 5-base 5′ overhang; heat-inactivated at 80°C for 20 min. | BglI |
60 fast-cut restriction enzymes
EcoRI
EcoRI fast-cut restriction endonuclease (recognition site G↓AATTC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
AflII
AflII fast-cut restriction endonuclease (recognition site C↓TTAAG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
ApaLI
ApaLI fast-cut restriction endonuclease (recognition site G↓TGCAC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
AvrII
AvrII fast-cut restriction endonuclease (recognition site C↓CTAGG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BamHI
BamHI fast-cut restriction endonuclease (recognition site G↓GATCC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BclI
BclI fast-cut restriction endonuclease (recognition site T↓GATCA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BglII
BglII fast-cut restriction endonuclease (recognition site A↓GATCT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BsaHI
BsaHI fast-cut restriction endonuclease (recognition site GR↓CGYC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BsiWI
BsiWI fast-cut restriction endonuclease (recognition site C↓GTACG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BspEI
BspEI fast-cut restriction endonuclease (recognition site T↓CCGGA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BsrFI
BsrFI fast-cut restriction endonuclease (recognition site R↓CCGGY) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BssHII
BssHII fast-cut restriction endonuclease (recognition site G↓CGCGC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
ClaI
ClaI fast-cut restriction endonuclease (recognition site AT↓CGAT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HindIII
HindIII fast-cut restriction endonuclease (recognition site A↓AGCTT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
KasI
KasI fast-cut restriction endonuclease (recognition site G↓GCGCC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
MluI
MluI fast-cut restriction endonuclease (recognition site A↓CGCGT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
MunI
MunI fast-cut restriction endonuclease (recognition site C↓AATTG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
NcoI
NcoI fast-cut restriction endonuclease (recognition site C↓CATGG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
NdeI
NdeI fast-cut restriction endonuclease (recognition site CA↓TATG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SalI
SalI fast-cut restriction endonuclease (recognition site G↓TCGAC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
XbaI
XbaI fast-cut restriction endonuclease (recognition site T↓CTAGA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
XhoI
XhoI fast-cut restriction endonuclease (recognition site C↓TCGAG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
PstI
PstI fast-cut restriction endonuclease (recognition site CTGCA↓G) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
AatII
AatII fast-cut restriction endonuclease (recognition site GACGT↓C) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
EcoT22I
EcoT22I fast-cut restriction endonuclease (recognition site ATGCA↓T) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
KpnI
KpnI fast-cut restriction endonuclease (recognition site GGTAC↓C) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SacII
SacII fast-cut restriction endonuclease (recognition site CCGC↓GG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
EcoRV
EcoRV fast-cut restriction endonuclease (recognition site GAT↓ATC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
DraI
DraI fast-cut restriction endonuclease (recognition site TTT↓AAA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
FspI
FspI fast-cut restriction endonuclease (recognition site TGC↓GCA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HpaI
HpaI fast-cut restriction endonuclease (recognition site GTT↓AAC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
MscI
MscI fast-cut restriction endonuclease (recognition site TGG↓CCA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
NaeI
NaeI fast-cut restriction endonuclease (recognition site GCC↓GGC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
NruI
NruI fast-cut restriction endonuclease (recognition site TCG↓CGA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
PmlI
PmlI fast-cut restriction endonuclease (recognition site CAC↓GTG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
PvuII
PvuII fast-cut restriction endonuclease (recognition site CAG↓CTG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
ScaI
ScaI fast-cut restriction endonuclease (recognition site AGT↓ACT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SmaI
SmaI fast-cut restriction endonuclease (recognition site CCC↓GGG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SnaBI
SnaBI fast-cut restriction endonuclease (recognition site TAC↓GTA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SspI
SspI fast-cut restriction endonuclease (recognition site AAT↓ATT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
AscI
AscI fast-cut restriction endonuclease (recognition site GG↓CGCGCC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
PmeI
PmeI fast-cut restriction endonuclease (recognition site GTTT↓AAAC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
SdaI
SdaI fast-cut restriction endonuclease (recognition site CCTGCA↓GG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BsaI
BsaI fast-cut restriction endonuclease (recognition site GGTCTC(N) ↓) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BspQI
BspQI fast-cut restriction endonuclease (recognition site GCTCTTC(N)↓) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
FokI
FokI fast-cut restriction endonuclease (recognition site GGATG(N)₉↓) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
DpnI
DpnI fast-cut restriction endonuclease (recognition site GAᵐ⁶↓TC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
DpnII
DpnII fast-cut restriction endonuclease (recognition site ↓GATC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
MboI
MboI fast-cut restriction endonuclease (recognition site ↓GATC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HaeIII
HaeIII fast-cut restriction endonuclease (recognition site GG↓CC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
AciI
AciI fast-cut restriction endonuclease (recognition site C↓CGC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
AluI
AluI fast-cut restriction endonuclease (recognition site AG↓CT) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HinfI
HinfI fast-cut restriction endonuclease (recognition site G↓ANTC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HinP1I
HinP1I fast-cut restriction endonuclease (recognition site G↓CGC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
HpaII
HpaII fast-cut restriction endonuclease (recognition site C↓CGG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
PspGI
PspGI fast-cut restriction endonuclease (recognition site ↓CCWGG) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
RsaI
RsaI fast-cut restriction endonuclease (recognition site GT↓AC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
TaqI
TaqI fast-cut restriction endonuclease (recognition site T↓CGA) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BstEII
BstEII fast-cut restriction endonuclease (recognition site G↓GTNACC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
BglI
BglI fast-cut restriction endonuclease (recognition site GCCNNNN↓NGGC) for site-specific DNA digestion within 5-15 min in the universal 10× Cut-Buffer.
Related categories
Documents
Manuals, COA and SDS are linked on each product row and on the product page.
Custom and OEM
Bulk fills, custom formulations and pilot evaluation are quoted per project.
Talk to an expert →Recognition sites at a glance
Top-strand recognition and cleavage site, end type, heat inactivation, reaction temperature and the methylation types the manual lists as blocking or affecting cleavage, for all 60 enzymes. Each product page carries the full methylation table, the number of sites in common DNA molecules and activity in other suppliers' buffers. To read a site or a primer tail on the opposite strand, paste it into our free reverse complement tool.
| Enzyme | Site (5′→3′) | Ends (overhang) | Heat inactivation | Reaction | Methylation | Price |
|---|---|---|---|---|---|---|
| AatII BR1G133 | GACGT↓C | 3′ (4 nt) | 80°C, 20 min | 37°C | CpG blocked | $45 30T |
| AciI BR1G102 | C↓CGC | 5′ (2 nt) | 80°C, 20 min | 37°C | CpG blocked | $50 25T |
| AflII BR1G153 | C↓TTAAG | 5′ (4 nt) | 80°C, 20 min | 37°C | — | $40 100T |
| AluI BR1G144 | AG↓CT | Blunt | 80°C, 20 min | 37°C | EcoBI blocked | $20 50T |
| ApaLI BR1G136 | G↓TGCAC | 5′ (4 nt) | 80°C, 20 min | 37°C | CpG blocked; EcoKI affected | $30 200T |
| AscI BR1G131 | GG↓CGCGCC | 5′ (4 nt) | 80°C, 20 min | 37°C | CpG affected | $40 50T |
| AvrII BR1G161 | C↓CTAGG | 5′ (4 nt) | No | 37°C | — | $55 25T |
| BamHI BR1G126 | G↓GATCC | 5′ (4 nt) | No | 37°C | — | $35 500T |
| BclI BR1G158 | T↓GATCA | 5′ (4 nt) | 80°C, 20 min | 37°C | Dam blocked; EcoBI blocked | $30 125T |
| BglI BR1G137 | GCCNNNN↓NGGC | 3′ (3 nt) | 80°C, 20 min | 37°C | CpG affected | $35 200T |
| BglII BR1G127 | A↓GATCT | 5′ (4 nt) | No | 37°C | EcoBI blocked | $20 100T |
| BsaHI BR1G159 | GR↓CGYC | 5′ (2 nt) | 80°C, 20 min | 37°C | Dcm overlap possible; CpG blocked; EcoBI overlap possible | $20 100T |
| BsaI BR1G110 | GGTCTC(N) ↓ | Type IIS | 80°C, 20 min | 37°C | Dcm affected; CpG affected | $40 50T |
| BsiWI BR1G156 | C↓GTACG | 5′ (4 nt) | 80°C, 20 min | 37°C | CpG blocked | $55 50T |
| BspEI BR1G106 | T↓CCGGA | 5′ (4 nt) | 80°C, 20 min | 37°C | Dam blocked; CpG affected | $20 50T |
| BspQI BR1G109 | GCTCTTC(N)↓ | Type IIS | 80°C, 20 min | 37°C | — | $40 50T |
| BsrFI BR1G107 | R↓CCGGY | 5′ (4 nt) | 80°C, 20 min | 37°C | CpG blocked | $20 50T |
| BssHII BR1G108 | G↓CGCGC | 5′ (4 nt) | 80°C, 20 min | 37°C | CpG blocked | $20 50T |
| BstEII BR1G130 | G↓GTNACC | 5′ (5 nt) | 80°C, 20 min | 37°C | EcoKI affected; EcoBI affected | $40 100T |
| ClaI BR1G129 | AT↓CGAT | 5′ (2 nt) | 80°C, 20 min | 37°C | Dam blocked; CpG blocked; EcoBI affected | $20 50T |
| DpnI BR1G122 | GAᵐ⁶↓TC | Blunt | 80°C, 20 min | 37°C | CpG affected; EcoBI affected | $40 50T |
| DpnII BR1G157 | ↓GATC | 5′ (4 nt) | 80°C, 20 min | 37°C | Dam blocked; EcoBI blocked | $20 50T |
| DraI BR1G134 | TTT↓AAA | Blunt | 80°C, 20 min | 37°C | EcoKI blocked | $40 100T |
| EcoRI BR1G111 | G↓AATTC | 5′ (4 nt) | 80°C, 20 min | 37°C | CpG affected; EcoBI affected | $45 600T |
| EcoRV BR1G149 | GAT↓ATC | Blunt | 80°C, 20 min | 37°C | CpG affected; EcoBI blocked | $35 200T |
| EcoT22I BR1G148 | ATGCA↓T | 3′ (4 nt) | 80°C, 20 min | 37°C | EcoBI affected | $20 25T |
| FokI BR1G143 | GGATG(N)₉↓ | Type IIS | 80°C, 20 min | 37°C | Dcm affected; CpG affected; EcoBI affected | $20 100T |
| FspI BR1G146 | TGC↓GCA | Blunt | No | 37°C | CpG blocked | $40 50T |
| HaeIII BR1G141 | GG↓CC | Blunt | 80°C, 20 min | 37°C | — | $40 300T |
| HindIII BR1G113 | A↓AGCTT | 5′ (4 nt) | 80°C, 20 min | 37°C | EcoBI blocked | $35 500T |
| HinfI BR1G138 | G↓ANTC | 5′ (3 nt) | 80°C, 20 min | 37°C | CpG affected; EcoBI blocked | $40 500T |
| HinP1I BR1G103 | G↓CGC | 5′ (2 nt) | 80°C, 20 min | 37°C | CpG blocked | $20 100T |
| HpaI BR1G135 | GTT↓AAC | Blunt | 80°C, 20 min | 37°C | CpG affected; EcoKI affected | $20 50T |
| HpaII BR1G105 | C↓CGG | 5′ (2 nt) | 80°C, 20 min | 37°C | CpG blocked | $40 200T |
| KasI BR1G154 | G↓GCGCC | 5′ (4 nt) | 80°C, 20 min | 37°C | CpG affected | $25 30T |
| KpnI BR1G162 | GGTAC↓C | 3′ (4 nt) | No | 37°C | — | $40 200T |
| MboI BR1G160 | ↓GATC | 5′ (4 nt) | 80°C, 20 min | 37°C | Dam blocked; CpG affected; EcoBI blocked | $20 50T |
| MluI BR1G121 | A↓CGCGT | 5′ (4 nt) | 80°C, 20 min | 37°C | CpG blocked; EcoKI affected; EcoBI affected | $80 100T |
| MscI BR1G117 | TGG↓CCA | Blunt | 80°C, 20 min | 37°C | Dcm blocked | $25 30T |
| MunI BR1G140 | C↓AATTG | 5′ (4 nt) | No | 37°C | — | $45 25T |
| NaeI BR1G112 | GCC↓GGC | Blunt | No | 37°C | CpG blocked | $20 50T |
| NcoI BR1G118 | C↓CATGG | 5′ (4 nt) | 80°C, 20 min | 37°C | — | $25 30T |
| NdeI BR1G119 | CA↓TATG | 5′ (2 nt) | 80°C, 20 min | 37°C | — | $40 200T |
| NruI BR1G164 | TCG↓CGA | Blunt | No | 37°C | Dam blocked; CpG blocked | $35 50T |
| PmeI BR1G139 | GTTT↓AAAC | Blunt | 80°C, 20 min | 37°C | CpG blocked | $45 50T |
| PmlI BR1G132 | CAC↓GTG | Blunt | 80°C, 20 min | 37°C | CpG blocked; EcoKI blocked; EcoBI blocked | $40 100T |
| PspGI BR1G163 | ↓CCWGG | 5′ (5 nt) | No | 75°C | Dcm blocked | $80 200T |
| PstI BR1G147 | CTGCA↓G | 3′ (4 nt) | No | 37°C | — | $40 500T |
| PvuII BR1G142 | CAG↓CTG | Blunt | No | 37°C | EcoBI affected | $30 200T |
| RsaI BR1G145 | GT↓AC | Blunt | No | 37°C | CpG affected | $40 100T |
| SacII BR1G125 | CCGC↓GG | 3′ (2 nt) | 80°C, 20 min | 37°C | CpG blocked | $20 50T |
| SalI BR1G115 | G↓TCGAC | 5′ (4 nt) | 80°C, 20 min | 37°C | CpG blocked | $75 200T |
| ScaI BR1G124 | AGT↓ACT | Blunt | 80°C, 20 min | 37°C | EcoBI blocked | $80 100T |
| SdaI BR1G152 | CCTGCA↓GG | 3′ (4 nt) | 80°C, 20 min | 37°C | — | $45 25T |
| SmaI BR1G128 | CCC↓GGG | Blunt | 80°C, 20 min | 37°C | CpG blocked | $40 100T |
| SnaBI BR1G151 | TAC↓GTA | Blunt | 80°C, 20 min | 37°C | CpG blocked | $20 60T |
| SspI BR1G150 | AAT↓ATT | Blunt | 80°C, 20 min | 37°C | — | $50 60T |
| TaqI BR1G155 | T↓CGA | 5′ (2 nt) | No | 65°C | Dam blocked | $40 200T |
| XbaI BR1G123 | T↓CTAGA | 5′ (4 nt) | 80°C, 20 min | 37°C | Dam blocked | $90 500T |
| XhoI BR1G116 | C↓TCGAG | 5′ (4 nt) | 80°C, 20 min | 37°C | CpG affected | $80 500T |
Frequently asked questions
Can two of these enzymes be used in the same reaction?
How long does a digestion take?
Which enzymes cannot be heat-inactivated?
Which products are in the Restriction Endonucleases category?
What documents are provided?
Can I order bulk packs or a custom formulation?
Need help choosing, or a custom format?
Send your workflow and requirements. We reply with a proposal and evaluation samples.
