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Fast qPCR · Cat. no. M2181-4

AcuGenix™ FastAmpli All-in-One qPCR Master Mix with UNG-V4

AcuGenix™ FastAmpli All-in-One qPCR Master Mix with UNG-V4, M2181-4, is supplied for human and animal related DNA detection.

Lyo-ready FM2181-4 Low-residual DNA M2181-4-NA Lyo-ready + Low-residual DNA FM2181-4-NA

≥ 1 kb / 10 s

Fast PCR in 25 min, ΔCt < 1 vs standard

3 copies / T

100% ASFV detection, Ct CV < 3%

20 freeze-thaw cycles

Stable amplification performance

AcuGenix™ FastAmpli All-in-One qPCR Master Mix with UNG-V4

AcuGenix™ FastAmpli Lyo-Ready All-in-One qPCR Master Mix with UNG-V4

AcuGenix™ FastAmpli All-in-One qPCR Master Mix with UNG (Low DNA)-V4

AcuGenix™ FastAmpli Lyo-Ready All-in-One qPCR Master Mix with UNG (Low DNA)-V4

Product overview

AcuGenix™ FastAmpli All-in-One qPCR Master Mix with UNG-V4 is a probe-based reagent specifically formulated for qualitative and quantitative Real-Time PCR applications. It incorporates a genetically engineered fast-amplification DNA polymerase capable of completing PCR amplification within 30 min.

Available versions

Cat. no.	Product name	Formulation	Residual DNA level
M2181-4	AcuGenix™ FastAmpli All-in-One qPCR Master Mix with UNG-V4	Liquid (with glycerol)	Standard
FM2181-4	AcuGenix™ FastAmpli Lyo-ready All-in-One qPCR Master Mix with UNG-V4	Lyo-ready	Standard
M2181-4-NA	AcuGenix™ FastAmpli All-in-One qPCR Master Mix with UNG (Low DNA)-V4	Liquid (with glycerol)	Low Residual DNA
FM2181-4-NA	AcuGenix™ FastAmpli Lyo-ready All-in-One qPCR Master Mix with UNG (Low DNA)-V4	Lyo-ready	Low Residual DNA

Features and applications

Features

Amplification rate is not less than 1kb/10s, which is suitable for purified DNA rapid fluorescence quantitative PCR, and the detection can be completed within 30 minutes at the fastest.
Excellent specificity and sensitivity.
More suitable for multiple amplification.

Applications

Human and animal related DNA detection.
Genomic DNA amplification and typing detection.
Fast amplification detection.

Components

Component	Specification/Notes
AcuGenix™ FastAmpli All-in-One qPCR Master Mix with UNG-V4	2×

Performance data

1. High- and Low-Template Balance (Amplification Efficiency)

Channel	FAM	VIC	ROX	CY5
M2181-4- Human Genomic Four-Plex Amplification
Amplification Curves

Conclusion 1: The M2181-4 system produced consistent amplification Rn plateaus across high-, medium-, and low-concentration templates, with excellent amplification curve profiles.

2. Fast Cycling Program Test

Standard PCR program	50°C 2min; 95°C 5min; 45 Cycles (95°C 10s, 55°C 40s)
Fast PCR program	50°C 2min; 95°C 30sec; 45 Cycles (95°C 1s, 60°C 8s)

Channel	FAM	VIC	ROX	CY5
M2181-4 -Standard PCR Program
Amplification Curves
M2181-4 - Fast PCR Program
Channel	FAM	VIC	ROX	CY5
Amplification Curves

Ct Value Statistics

Channel	Template Concentration (pg/μL)	Standard PCR Program	Fast PCR Program	Fast- Standard Program (ΔCt)
FAM	25	29.45	29.98	0.53
FAM	250	26.26	26.73	0.47
VIC	25	30.07	30.57	0.5
VIC	250	26.54	27.24	0.7
ROX	25	31.04	31.42	0.38
ROX	250	28.12	28.44	0.32
CY5	25	29.17	29.36	0.19
CY5	250	25.82	25.98	0.16

Conclusion 1: M2181-4 is compatible with the fast PCR program. Across all channels, the mean amplification ΔCt for fast PCR program (25 min) versus standard PCR cycling (70 min) was less than 1.

3. Sensitivity and Precision Test

Channel	FAM (Target)	VIC (Internal Control)
M2181-4 - Low-Concentration ASFV Plasmid Template Amplification
Amplification Curves

Sample	ASFV plasmid template, 3 copies/T
Target Ct Value	37.58	38.51	37.52	37.53
	38.64	37.32	35.71	36.85
	35.65	36.88	35.59	36.12
	38.39	37.53	36.40	36.95
	36.79	35.48	36.52	35.78
	37.86	36.63	38.33	36.51
Mean	36.96
SD	0.95
Ct CV%	2.58%
Detection Rate	100%

Conclusion 1: The M2181-4 reagent achieved 100% detection of African swine fever virus (ASFV) plasmid template at the detection limit of 3 copies/T, with a Ct CV of less than 3%.

4. Freeze-Thaw Stability Test

Channel	FAM	VIC	ROX	CY5
0 Freeze-Thaw Cycles; 5 Freeze-Thaw Cycles; 10 Freeze-Thaw Cycles; 15 Freeze-Thaw Cycles; 20 Freeze-Thaw Cycles
Amplification Curves

Conclusion 1: The M2181-4 premix reagent, after 5, 10, 15, and 20 freeze-thaw cycles, showed amplification performance consistent with that of the non-freeze-thawed reagent and remained stable.

5. Accelerated Stability Test

Channel	FAM	VIC	ROX	CY5
0 Days Accelerated; 3 Days Accelerated; 7 Days Accelerated; 14 Days Accelerated
Amplification Curves

Conclusion 1: The M2181-4 premix reagent, after accelerated storage at 37°C for 7 and 14 days, showed amplification performance consistent with that of the non-accelerated reagent and remained stable.