Sensi Direct All-in-One qPCR Master Mix with UNG

Sensi Direct All-in-One qPCR Master Mix with UNG, MD2091, is supplied for detection of DNA viruses related to humans and animals.

Lyo-ready FMD5091

3 copies / T

100% ASFV detection, Ct CV < 3%

~99% efficiency

R² 0.998–0.999 on HBV assay

Plasma, serum, saliva

Extraction-free inhibitor tolerance

Sensi Direct All-in-One qPCR Master Mix with UNG

Sensi Direct Lyo-Ready All-in-One qPCR Master Mix with UNG (Low DNA)

Product overview

Sensi Direct All-in-One qPCR Master Mix with UNG is a specialized qPCR reagent designed for direct quantitative detection of target genes using a probe-based fluorescence detection, bypassing the nucleic acid extraction and purification steps.

Available versions

Cat. no.	Product name	Formulation
MD2091	Sensi Direct All-in-One qPCR Master Mix with UNG	Liquid (with glycerol)
FMD5091	Sensi Direct Lyo-ready All-in-One qPCR Master Mix with UNG	Lyo-ready

Features and applications

Features

Has good tolerance to inhibitors from serum, saliva, etc.
The amplification rate is not less than 1kb/10s, suitable for rapid extraction-free fluorescence quantitative PCR, completion of detection within 30 minutes at the fastest.

Applications

Detection of DNA viruses related to humans and animals.
Genomic DNA amplification and typing detection.

Components

Component	Specification/Notes
Sensi Direct All-in-One qPCR Master Mix with UNG	2×

Performance data

1. High/Low Template Balance

Channel	FAM (Target)	VIC (Internal Control)
MD2091 System - HBV Assay Amplification
Amplification Curves
Standard Curve

Conclusion 1: In the HBV assay, MD2091 produced consistent amplification-curve Rn plateaus across high-, medium-, and low-concentration templates, with an amplification efficiency of approximately 99% and an R² coefficient of 0.998-0.999.

2. Sensitivity and Precision Test

Channel	FAM (Target)
MD2091 - ASFV Assay - Low-Concentration ASFV Plasmid Template Amplification
Amplification Curves

Sample	ASFV plasmid template, 3 copies/T
Target Ct Value	38.45	35.97	35.65	36.39
	38.05	36.88	34.97	36.00
	36.32	36.13	36.12	36.44
	37.39	36.10	35.73	36.51
	36.04	38.12	34.77	36.06
	37.00	36.10	36.99	37.20
Mean	36.47
SD	0.89
Ct Value CV%	2.44%
Detection Rate	100%

Conclusion 1: MD2091 detected the African swine fever virus (ASFV) plasmid template at the 3 copies/T detection limit with a 100% detection rate, and the Ct value CV was less than 3%.

3. Plasma and Serum Inhibitor-Tolerance Test

Channel	FAM (Target)	VIC (Internal Control)
MD2091 System - HBV Assay Amplification
HBV Purified Template
HBV Plasma Template
HBV Serum Template

Template Name	Sample Concentration (IU/ml)	Equivalent Template Concentration (IU/T)	Mean Ct Value
Purified HBV Template	10⁴	235	28.72
	10³	23.5	31.90
	50	1.2	35.78
HBV Plasma Template	1.6×10⁴	100	30.17
	1.6×10³	10	33.45
	1.6×60	0.6	37.90
HBV Serum Template	1.6×10⁴	100	29.87
	1.6×10³	10	33.17
	1.6×60	0.6	38.68

Conclusion 1: Based on the calculated template equivalents, the amplification efficiencies of the HBV plasma/serum templates and the purified template were comparable, indicating that MD2091 had good tolerance to plasma and serum.

4. Saliva Inhibitor-Tolerance Test

Channel	FAM	VIC	ROX	CY5
Conventional Product MD2091
Amplification Curves

Conclusion 1: When saliva samples were used as templates, MD2091 consistently detected the target human genomic DNA template. Compared with the conventional product, MD2091 performed better in the direct amplification of saliva samples and was highly suitable for direct amplification of DNA from saliva samples.