AcuGenix™ FastAmpli RT-qPCR Master Mix with UNG
AcuGenix™ FastAmpli RT-qPCR Master Mix with UNG, M5144, is suitable for multiplex multi-channel RT-qPCR.
Product overview
AcuGenix™ FastAmpli RT-qPCR Master Mix with UNG is a specialized reagent for fluorescence quantitative (TaqMan probe-based) RNA amplification assays, containing genetically engineered reverse transcriptase and DNA polymerase optimized for rapid amplification, enabling completion of PCR reactions within 20–40 minutes.
Available versions
| Cat. no. | Product name | Formulation | Residual DNA level |
|---|---|---|---|
| M5144 | AcuGenix™ FastAmpli RT-qPCR Master Mix with UNG | Liquid (with glycerol) | Standard |
| FM5144 | AcuGenix™ FastAmpli Lyo-ready RT-qPCR Master Mix with UNG | Lyo-ready | Standard |
| M5144-NA | AcuGenix™ FastAmpli RT-qPCR Master Mix with UNG (Low DNA) | Liquid (with glycerol) | Low Residual DNA |
| FM5144-NA | AcuGenix™ FastAmpli Lyo-ready RT-qPCR Master Mix with UNG (Low DNA) | Lyo-ready | Low Residual DNA |
Features and applications
- Suitable for high-sensitivity amplification of low-concentration RNA templates.
- Rapid amplification
- High detection sensitivity.
- Suitable for multiplex multi-channel RT-qPCR.
- Suitable for high-sensitivity amplification of low-concentration RNA templates.
- Suitable for fast amplification.
Components
| Component | Specification/Notes |
|---|---|
| 10×FastAmpli RTase/UNG Mix | 10× |
| 5×FastAmpli RT Premix Buffer (dUTP) (Mg2+ free) (Glycerol-Free) | 5× |
| 25×MgCl2 | 25× |
Performance data
1. Accelerated Stability Test
| M5144 System - Human Total RNA Triplex Amplification Accelerate 0 Day Accelerate 7 Days Accelerate 14 Days | |||
| Channel | VIC | ROX | CY5 |
|---|---|---|---|
| Amplification Curves |  |  |  |
Conclusion 1: After M5144 was held at 37°C under accelerated-aging conditions for 7 and 14 days, its amplification performance was consistent with that of the non-aged reagent, demonstrating maintained stability.
2. Freeze-Thaw Stability Test
| M5144 System - Human Total RNA 0 Freeze-Thaw Cycle 5 Freeze-Thaw Cycles 10 Freeze-Thaw Cycles 15 Freeze-Thaw Cycles 20 Freeze-Thaw Cycles | ||||
| Channel | FAM | VIC | ROX | CY5 |
|---|---|---|---|---|
| Amplification Curves | / |  |  |  |
Conclusion 1: After 5, 10, 15, and 20 freeze-thaw cycles, the amplification performance is consistent with that of the reagent without freeze-thaw treatment, indicating that it remains stable after 20 freeze-thaw cycles.
3. Rapid Amplification Test
| M5144 System Fast PCR program: 50°C 5min; 95°C 1min; 50 Cycles (95°C 1s, 60°C 10s) | ||
| Channel | FAM | VIC |
|---|---|---|
| Amplification Curve |  |  |
Conclusion 1: Under the rapid amplification procedure, M5144 detected HCV templates, achieving full detection at high, medium, and low concentrations of the templates, with normal amplification curves, indicating that this system is suitable for rapid amplification.
4. Amplification Efficiency Test
| M5144-Human Total RNA | ||
| Channel | Amplification Curves | standard curve |
|---|---|---|
| CY5 |  |  |
| Correlation Coefficient | Amplification Efficiency |
|---|---|
| 0.99924 | 99.95% |
Conclusion 1: The M5144 reagent showed excellent linearity across a broad range of template concentrations, with good amplification efficiency and reproducible, reliable quantitative results.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Conditions | -20±5°C |
| Storage Conditions | -20±5°C |
| Shelf Life | 18M |
Downloads
AcuGenix™ FastAmpli RT-qPCR Master Mix with UNG (M5144)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)MSDS 3 (PDF)AcuGenix™ FastAmpli Lyo-ready RT-qPCR Master Mix with UNG (FM5144)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)MSDS 3 (PDF)AcuGenix™ FastAmpli RT-qPCR Master Mix with UNG (Low DNA) (M5144-NA)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)MSDS 3 (PDF)AcuGenix™ FastAmpli Lyo-ready RT-qPCR Master Mix with UNG (Low DNA) (FM5144-NA)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)MSDS 3 (PDF)
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