Melt Curve RT-qPCR · Cat. no. M8254
Neoscript Multi RT-qPCR Master Mix with UNG
Neoscript Multi RT-qPCR Master Mix with UNG, M8254, is supplied for probe-based melting curve genotyping detection, e.g., respiratory virus multiplex detection.
One-step multiplex
RT-qPCR with UNG
Melt-curve genotyping
Probe-based respiratory panels
Product overview
Neoscript Multi RT-qPCR Master Mix with UNG is a dedicated reagent for one-step qualitative and quantitative RT-qPCR using the probe method and is suitable for multiplex amplification. The entire reaction is performed sequentially in a single tube, avoiding tube opening and effectively reducing contamination.
Available versions
| Cat. no. | Product name | Formulation | Residual DNA level |
|---|---|---|---|
| M8254 | Neoscript Multi RT-qPCR Master Mix with UNG | Liquid (with glycerol) | Standard |
| FM8254 | Neoscript Lyo-ready Multi RT-qPCR Master Mix with UNG | Lyo-ready | Standard |
| M8254-NA | Neoscript Multi RT-qPCR Master Mix with UNG (Low DNA) | Liquid (with glycerol) | Low Residual DNA |
| FM8254-NA | Neoscript Lyo-ready Multi RT-qPCR Master Mix with UNG (Low DNA) | Lyo-ready | Low Residual DNA |
Features and applications
Features
- One-step multiplex reverse-transcription quantitative real-time PCR.
- Designed for sensitive amplification of low-concentration RNA templates.
Applications
- Probe-based melting-curve genotyping assays, including multiplex respiratory-virus detection.
Components
| Component | Specification / Notes |
|---|---|
| 20×Neoscript RTase/UNG Multi Mix | N/A |
| 8×Neoscript RT Premix Multi Buffer (dUTP) (Mg2+ free) (DG) | Included component; refer to the instruction manual |
| 360mM MgCl2 | Included component; refer to the instruction manual |
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Temperature | Ice pack transportation (2-8°C) |
| Storage Temperature | -20±5°C |
| Shelf Life | 12M |
Downloads
Neoscript Multi RT-qPCR Master Mix with UNG (M8254)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)MSDS 3 (PDF)Neoscript Lyo-ready Multi RT-qPCR Master Mix with UNG (FM8254)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)MSDS 3 (PDF)Neoscript Multi RT-qPCR Master Mix with UNG (Low DNA) (M8254-NA)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)MSDS 3 (PDF)Neoscript Lyo-ready Multi RT-qPCR Master Mix with UNG (Low DNA) (FM8254-NA)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)MSDS 3 (PDF)
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