AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-V2
AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-V2, E04-2, is supplied for contamination control.
Product overview
AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-V2 is a temperature-sensitive UNG enzyme recombinantly expressed in Escherichia coli. This enzyme catalyzes the release of free uracil from uracil-containing single-stranded and double-stranded DNA, and exhibits no activity against RNA.
Available versions
| Cat. no. | Product name | Formulation | Residual DNA level |
|---|---|---|---|
| E04-2 | AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-V2 | Liquid (with glycerol) | Standard |
| FE04-2 | AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-V2 (Glycerol-Free) | Glycerol-free | Standard |
| E04-2-NA | AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-Low DNA-V2 | Liquid (with glycerol) | Low Residual DNA |
| FE04-2-NA | AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-Low DNA (Glycerol-Free)-V2 | Glycerol-free | Low Residual DNA |
Features and applications
- It maintains higher activity at low temperatures ranging from 20°C to 37°C. It is heat-labile and can be efficiently inactivated at 50°C, which prevents the degradation of dUTP-containing amplicons at room temperature caused by residual activity of conventionally inactivated UNG.
- This product is recommended for anti-contamination applications in conventional PCR and RT-PCR assays.
- UNG/dUTP anti-contamination system.
Components
| Component | Specification/Notes |
|---|---|
| AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-V2 | 1 U/μL |
Performance data
1. Digestive capacity Test
| Positive control Negative controlHeat-labile UNG with dUTP-containing template at 37°C for 10 minutes The U-containing template: 103 copies/T | |
| Channel | FAM |
|---|---|
| Amplification Curves |  |
Conclusion 1: After 10 minutes of treatment at 37°C, the U-containing template below 103 was completely degraded without producing amplification products.
2. Inactivation capacity Test
| Positive control Heat-labile UNG at 50°C for 10 minutes | |
| Channel | FAM |
|---|---|
| Amplification Curves |  |
Conclusion 1: After 10 minutes of treatment at 50°C, the activity of Heat-labile UNG is inactivated.
3. Validity verification
| Digestive capacity Test Positive control Heat-labile UNG with dUTP-containing template at 37°C for 10 minutes | |
| Channel | FAM |
|---|---|
| Instant production |  |
| Store at -20 ± 5 °C for 2 years |  |
Conclusion 1: E04-2 exhibits good stability when stored at -20 ± 5 °C for 2 years.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Conditions | -20±5°C |
| Storage Conditions | -20±5°C |
| Shelf Life | 24M |
Downloads
AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-V2 (E04-2)
Instruction Manual (PDF)COA (PDF)MSDS (PDF)AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-V2 (Glycerol-Free) (FE04-2)
Instruction Manual (PDF)COA (PDF)MSDS (PDF)AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-Low DNA-V2 (E04-2-NA)
Instruction Manual (PDF)COA (PDF)MSDS (PDF)AcuGenix™ Heat-labile Uracil-DNA Glycosylase (UNG)-Low DNA (Glycerol-Free)-V2 (FE04-2-NA)
Instruction Manual (PDF)COA (PDF)MSDS (PDF)
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