AcuGenix™ Hot Start LAMP Dye Master Mix-V2
AcuGenix™ Hot Start LAMP Dye Master Mix-V2, HW205-R02, is supplied for molecular diagnostic research and manufacturing workflows.
Product overview
Loop mediated isothermal amplification (LAMP) is a nucleic acid amplification technique. This kit uses dye based isothermal amplification and can be used for qualitative detection of DNA in samples such as viruses.
Available versions
| Cat. no. | Product name | Formulation |
|---|---|---|
| HW205-R02 | AcuGenix™ Hot Start LAMP Dye Master Mix-V2 | Liquid (with glycerol) |
| FHW1007-R02 | AcuGenix™ Lyo-ready Hot Start LAMP Dye Master Mix-V2 | Lyo-ready |
Features and applications
- Suitable for DNA sample detection by LAMP using intercalating dyes, requiring fluorescence detection instruments.
- Reactions can be assembled at room temperature, effectively preventing non-specific amplification and enhancing reaction efficiency.
- This kit employs a dye-based isothermal amplification method.
- This kit does not contain glycerol components, making it suitable for those with lyophilization conditions to develop lyophilized reagents.
Components
| Component | Specification/Notes |
|---|---|
| Liquid (with glycerol), HW205-R02 | |
| AcuGenix™ Bst Hot Start DNA Polymerase-V2 (8 U/µL) | N/A |
| 2×LAMP Premix Buffer Ⅱ | Included component; refer to the instruction manual |
| 50×LAMP Fluorescent Dye Ⅱ | Included component; refer to the instruction manual |
| Lyo-ready, FHW1007-R02 | |
| 10×LAMP Premix (DG) (Bst2.0 HS) | 10× |
| 5×LAMP Lyophilization Buffer | 5× |
| 50×LAMP Fluorescent Dye Ⅱ | 50× |
Performance data
1. Accelerated Stability Test
| Stored at -20°C Accelerated at 37°C for 7 d Accelerated at 37°C for 14 d | ||||
| Channel | 2ng/μl | 0.2ng/μl | 0.02ng/μl | NTC |
|---|---|---|---|---|
| FAM |  |  |  |  |
NTC Testing Standard:NTC amplification start time >30 min;
Conclusion 1: FHW1007-R02 remains stable after being accelerated at 37°C for 7 and 14 days.
2. Freeze-Thaw Stability Test
| Stored at -20°C 5 freeze-thaw cycles 10 freeze-thaw cycles 15 freeze-thaw cycles | ||||
| Channel | 2ng/μl | 0.2ng/μl | 0.02ng/μl | NTC |
|---|---|---|---|---|
| FAM |  |  |  |  |
NTC Testing Standard:NTC amplification start time >30 min;
Conclusion 1: FHW1007-R02 remained stable after 15 freeze-thaw cycles.
3. Immediate Post-Lyophilization Test
All lyophilized beads were spherical, with smooth surfaces and no cracking, shrinkage, or melting.
| ■ Liquid Control ■ Lyophilized Beads | ||||
| Channel | 0.1 pg/μl | 0.01 pg/μl | 0.001 pg/μl | NTC |
|---|---|---|---|---|
| FAM |  |  |  |  |
NTC Testing Standard:NTC amplification start time >30 min;
Conclusion 1: The FHW1007-R02 lyophilised balls look normal after freeze-drying and perform just as well as the liquid control.
Shipping and storage
| Condition | Recommendation |
|---|---|
| Shipping Conditions | -20±5°C |
| Storage Conditions | -20±5°C |
| Shelf Life | 18M |
Downloads
AcuGenix™ Hot Start LAMP Dye Master Mix-V2 (HW205-R02)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)MSDS 3 (PDF)AcuGenix™ Lyo-ready Hot Start LAMP Dye Master Mix-V2 (FHW1007-R02)
Instruction Manual (PDF)COA (PDF)MSDS 1 (PDF)MSDS 2 (PDF)MSDS 3 (PDF)
Need this product lyophilized, in bulk, or in a custom format?
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