AcuGenix™ Hot Start LAMP Probe Master Mix-V2

AcuGenix™ Hot Start LAMP Probe Master Mix-V2, HW205-P01, is supplied for molecular diagnostic research and manufacturing workflows.

Lyo-ready FHW1007-P01

1 year at RT

Lyophilized form performs like the liquid control

Room-temp setup

Prevents non-specific amplification

AcuGenix™ Hot Start LAMP Probe Master Mix-V2

Product overview

AcuGenix™ Lyo-ready Hot Start LAMP Probe Master Mix-V2 is specifically developed for the lyophilization process, utilizing the probe method for qualitative and quantitative detection of DNA in samples. AcuGenix™ Bst Hot Start DNA Polymerase-V2 in the product is derived from Bacillus stearothermophilus DNA Polymerase, exhibiting 5’→3’ DNA polymerase activity and strong strand displacement capability, while lacking 3’→5’ exonuclease activity. Compared to the wild-type Bst DNA polymerase large fragment, this enzyme effectively enhances amplification speed and yield. Obtained through reversible modification technology based on AcuGenix™ Bst DNA Polymerase-V2, AcuGenix™ Bst Hot Start DNA Polymerase-V2 can completely inhibit enzyme activity at room temperature, enabling reaction establishment at room temperature, preventing nonspecific amplification, and improving reaction efficiency. Additionally, AcuGenix™ Bst Hot Start DNA Polymerase-V2 does not require a separate activation step. This reagent utilizes an optimized formula of LAMP probe method lyophilization-specific Buffer, suitable for preparing full-component lyophilized products, such as lyophilized spheres and in-situ lyophilized powders.

Available versions

Cat. no.	Product name	Formulation
HW205-P01	AcuGenix™ Hot Start LAMP Probe Master Mix-V2	Liquid (with glycerol)
FHW1007-P01	AcuGenix™ Lyo-ready Hot Start LAMP Probe Master Mix-V2	Lyo-ready

Features and applications

Features

Designed for probe-based LAMP detection of DNA samples, requiring instrumentation for fluorescence readout.
Reactions can be assembled at room temperature, effectively preventing non-specific amplification and enhancing reaction efficiency.

Applications

Molecular diagnostic research and manufacturing workflows.

Components

Component	Specification/Notes
Liquid (with glycerol), HW205-P01
AcuGenix™ Bst Hot Start DNA Polymerase-V2 (8 U/µL)	N/A
5×LAMP Premix Buffer Ⅱ	Included component; refer to the instruction manual
Lyo-ready, FHW1007-P01
10×LAMP Premix (DG) (Bst2.0 HS) (RNase H II)	10×
5×LAMP Lyophilization Buffer	5×

Performance data

1. Immediate Post-Lyophilization Test

FHW1007-P01 AcuGenix™ Lyo-ready Hot Start LAMP Probe Master Mix-V2: Immediate Post-Lyophilization Test

Lyophilized Appearance: All lyophilized beads were spherical with smooth surfaces and showed no cracking, shrinkage, or melting.

Channel	2ng/μl	0.2ng/μl	0.02ng/μl	NTC
Liquid Control Lyophilized Beads
FAM

Conclusion 1: No significant performance difference was observed between lyophilized FHW1007-P01 and the liquid control.

2. One-Year Stability Test at Room Temperature After Lyophilization

The lyophilized product was conical and showed no cracking, shrinkage, or melting.

All lyophilized beads were spherical with smooth surfaces and showed no cracking, shrinkage, or melting.

Channel	2ng/μl	0.2ng/μl	0.02ng/μl	NTC
Immediate Liquid Control In-Situ Lyophilized, Stored at Room Temperature for One Year Lyophilized Beads, Stored at Room Temperature for One Year
FAM

Conclusion 1: After being left at room temperature for a year, the original freeze-dried samples and freeze-dried spheres were normal in shape and their amplification performance was consistent with the liquid control.